Investigation of flowing time, flower structure, microspore density, microspore vitality and microspore-derived embryo (MDE) formation rate according to the light quality treatment on broccoli donor plant was accomplished. The material was 08-8-3 line yielding high MDE production rate having 4.0 ± 0.5 mm flower bud length. The donor plant was cultivated with light quality treatment of red LED light, red+blue+white LED light and fluorescent light. The light intensity was 50 μ molm-2s-1 and photoperiod was 16/8 hours (light/dark). The flowering time was fastest at red LED light treatment compared to the other light treatment condition. 100.0, 36.4 and 18.2% of flower bud with longer stigma length than floral leaf which reported high MDE production rate were found under red LED light, Red+Blue+White LED light and fluorescent lights respectively. The microspore density and MDE production rate per single flower bud was highest at Red LED light. Suitable flower bud and high MDE production rate could be achieved in a short period if using LED light to broccoli donor plant cultivation. The above result is thought to be very useful for the development of a new cultivar of broccoli and other many crops including Brassica using haploid breeding technology. This journal was supported by the National Research Foundation