본 연구에서는 유도가열기법을 활용한 콘크리트 촉진 발열양생 기술의 효율성을 평가하기 위하여 철근 배근 및 전도체 변수에 따른 유도가열에 의한 콘크리트 온도향상 성능 및 압축강도를 평가하였 다. 먼저 철근 자체의 유도가열 성능을 평가한 결과, 주철근 및 나선철근 모두 유도가열 직후 10분 이 내에 목표온도인 70℃에 도달하여 충분한 가열성능을 나타내었다. 주요변수로 나선철근의 유무, 나선 철근의 간격, 피복두께, 거푸집 종류, 시험체 크기의 변수를 고려하여 15시간의 유도가열 발열양생 후 압축강도 평가를 실시하였다. 평가 결과, 철근을 포함한 경우 유도가열 발열양생 효율이 약 20~85% 만큼 증가하였다. 또한 총 6종(강섬유, 비정질강섬유, 전기로슬래그, 탄소섬유, 탄소나노튜브, 흑연나노 섬유)의 전도성 재료를 선정하여 재료 자체의 유도가열을 통해 발열성능을 평가하였다. 평가결과 강섬 유, 비정질강섬유, 전기로슬래그는 1~7분 사이에 가열 목표온도인 70℃에 근접하게 도달하여 유도가 열에 의한 콘크리트 발열성능을 향상 시킬 수 있는 혼입재료로 최종 선정하였다. 이상의 3가지 전도체 를 혼입하여 콘크리트를 타설한 뒤 15시간 유도가열 후의 압축강도를 평가한 결과, 강섬유 1%를 혼 입한 경우 발열양생을 실시하지 않은 상온양생에 비하여 강도가 186% 증가하였고, 잔골재의 50%를 전기로 슬래그로 치환한 경우 발열양생을 실시하지 않은 상온양생에 비하여 강도가 187% 상승하였다.

Considerable attention has been focused on the cryopreservation of semen and estrus induction in dog, as consequence of poor productivity caused by long anestrus period, in order to enhance the productivity of youngs and to preserve the breeds. The objectives of this study were to evaluate semen quality after cryopreservation and to evaluate the Pregnancy rate after insemination (AI). Fifty infertilie dogs (age 2∼3 years) were selected for the study and divided into three different estrus induction treatment groups. Group 1: dogs (n=15) were given clomifene (0.1 mg/kg) orally for five days at 12 hr intervals. Group 2: dogs (n=15) were given bromocriptine (50 /kg) orally for five days at 12 hr intervals, followed by single injection intravenously of 500 IU GnRH (Group 3, n=20) when pro-estrus occurred. The rates of pregnancy in estrus inducted dogs mated naturally compared to those inseminated artificially with ejaculated fresh semen and frozen-thawed semen. Estrus detection was performed using the method of vaginal smear and confirmed by the plasma progesterone assay. The ejaculated semen to freeze was exposed to a mixture of Tris extender with cryoprotectant (Trisma, 81 mM: TES, 209 mM: citric acid, 6 mM; glucose, 5 mM; glycerol, 8%) and cryopreserved gradually by slow-cooling at 17 cm above the surface of liquid nitrogen (LN) for 23 min. The motility of frozen-thawed spermatozoa was assessed by phase-contrast microscopy. To assess their viability and acrosome content, spermatozoa were stained with a vital stain and Fluorescence conjugated lectin Pisum Savitum Agglutinin (FITC/PAS), respectively. Pregnancy was confirmed by ultrasonograpy on day 25, 35 and 55 post insemination. The use of fresh semen, the pregnancy rates were observed 66.6, 66.6, 75.0 and 83.3% in natural estrus, clomifene induced, bromocriptine induced and a combination of GnRH and bromocriptine, respectively. The use of frozen-thawed semen, the pregnancy rates were observed 66.6, 33.3, 50.0 and 60.0% in natural estrus, clomifene induced, bromocriptine induced and a combination of GnRH and bromocriptine, respectively. No difference was observed in the number of offspring produced among natural estrus and treated groups inseminated with fresh or frozen-thawed semen. In conclusion, the pregnancy rate of dogs treated with a combination of GnRH and bromocriptine was more effective than use of clomifene or bromocriptine only. In addition, frozen-thawed semen can be used successfully far artificial insemination in dog.

Considerable attention has been focused on the cryopreservation of semen and estrus induction in dog, as consequence of poor productivity caused by long anestrus period, in order to enhance the productivity of youngs and to preserve the breeds. The objectives of this study were to develop a treatment protocol for estrus induction. Fifty infertilie dogs (age 2∼3 years) were selected for the study and divided into three different estrus induction treatment groups. Group 1: dogs (n=15) were given clomifane (0.1 mg/kg) orally for five days at 12 hr intervals. Group 2: dogs (n=15) were given bromocriptine (50 /kg) orally for five days at 12 hr intervals, followed by single injection intravenously of 500 IU GnRH (Group 3, n=20) when pro-estrus occurred. After being treated, the dogs were evaluated for the rates of estrus induction and time interval lapses from treatment to beginning of the pro-estrus. The estrus induction rates were significantly (P<0.05) higher in both group 2 (9/15, 73.3%) and group 3 (16/20, 80.0%) than that of group 1 (9/15, 60.0%), but did not differ in the groups 2 and 3. No differences were observed in the time interval lapses from treatment to beginning of the pro-estrus in group 2 (7.7 1.2 days) and group 3 (6.9 2.0 days), but significantly (P<0.05) shorter than that of group 1 (9.5 2.1 days). In conclusion, the estrus induction rate of dogs treated with a combination of GnRH and bromocriptine was more effective than use of clomifene or bromocriptine only.

The effect of carbenicillin on the dedifferentiation and the regeneration efficiency of plant tissues of horseradish(Armoracia rusticana) was evaluated, Inhibition effect for callus initiation was observed when leaf blade, root and petiole segments were grown on MS medium containing 500 mg/L to 2000 mg/L carbenicillin and 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The regeneration of horseradish shoots from leaf blade, root and petiole explants were decreased as the addition of carbenicillin increased from 1000 mg/L to 2000 mg/L in MS medium containing 0.5 mg/L of 6-benzylaminopurine (BAP) or kinetin. Especially, 500 mg/L carbenicillin treatment significantly inhibited shoot induction when leaf blade explants were grown on hormone-free MS medium. It was suggested that the toxic effects of combinations of carbenicillin and 2,4-D may be due to high auxin activity levels.