본 연구에서는 와편모조류 Alexandrium affine(LIMS-PS-2345)의 생장에 미치는 용존태 무기 및 유기 영양염의 영향을 조사하였 다. 영양염 흡수 동력학 실험에서 A. affine의 최대흡수속도(ρmax)와 반포화상수(Ks)는 질산염에서 77.0 pmol/cell/hr과 17.6 μM, 인산염에서 15.5 pmol/cell/hr과 3.88 μM로 산출되어, 무기영양염에 대하여 높은 요구량 및 낮은 친화성을 가지고 있는 것으로 나타났다. 유기 영양염에 따른 A. affine의 생장속도를 확인한 결과, 유기 질소 urea, glycine와 유기 인 adenosine triphosphate(ATP), glycerol phosphate(Glycerol-P) 첨가구에서 무기 영양염 첨가구의 70 % 이상 생장속도를 보였다. 따라서 낮은 무기 영양염 환경에서 A. affine의 우점화와 종간경쟁에서 우위를 위해서는 용존태 유기 영양염의 이용이 필요할 것으로 생각된다.

Biological phosphorus removal is accomplished by exposing PAO(phosphorus accumulating organisms) to anaerobic-aerobic conversion conditions. In the anaerobic condition, PAO synthesize PHB(polyhydroxybutyrate) and simultaneously hydrolysis of poly-p resulting phosphorus(Pi) release. In aerobic condition, PAO uptake phosphorus(Pi) more than they have released. In this study, cyanobacteria Synechococcus sp., which is known to be able to synthesize PHB like PAO, was exposed to anaerobic-aerobic conversion. If Synechococcus sp. can remove excess phosphorus by the same mechanism as PAO, synergistic effects can occur through photosynthesis. Moreover, Synechococcus sp. is known to be capable of synthesizing PHB using inorganic carbon as well as organic carbon, so even if the available capacity of organic carbon decreases, it was expected to show stable phosphorus removal efficiency. In 6 hours of anaerobic condition, phosphorus release occurred in both inorganic and organic carbon conditions but SPRR(specific phosphorus release rate) of both conditions was 10 mg-P/g-MLSS/day, which was significantly lower than that of PAO. When converting to aerobic conditions, SPUR(specific phosphorus uptake rate) was about 9 mg-P/g-MLSS/day in both conditions, showing a higher uptake rate than the control condition showing SPUR of 6.4 mg-P/g-MLSS/day. But there was no difference in terms of the total amount of removal. According to this study, at least, it seems to be inappropriate to apply Synechococcus sp. to luxury uptake process for phosphorus removal.

The objective of this study was to quantify and characterize the inorganic nitrogen and phosphorus outflow loading from different water managements in paddy fields. We investigated the NO3-N, NH4-N, and PO4 in runoff from paddy fields in Iksan. The three different water management treatments were conventional continuous irrigation at 4 and 8 cm water levels, and intermittent irrigation at a 4 cm water level. The concentration of NO3-N at the early growth stage in surface water was 6.11 mg L-1, and then it gradually decreased. The downward curve increased slightly with additional nitrogen fertilization at the panicle initiation stage, and then it continued to decrease. The NH4-N concentration was 5.26 mg L-1, and that of PO4 was 0.70 mg L-1 at the early growth stage. However, the concentration of NO3-N peaked at 8.79 mg L-1 directly after transplantation and then decreased rapidly throughout the growing season. The amount of NH4-N runoff was 1.86 kg ha-1 in the plot with intermittent irrigation, and 2.0 kg ha-1 and 2.1 kg ha-1 in the plots with water depths of 4 and 8 cm, respectively. The NO3-N runoff was 7.43 kg ha-1 in the plot with intermittent irrigation, 8.62 kg ha-1 in the plot with a water depth of 4 cm, and 10.25 kg ha-1 in the plot with a water depth of 8 cm. In addition, the PO4 runoff was 0.42 kg ha-1 in the plot with intermittent irrigation, 0.48 and 0.55 kg ha-1 in the plots with water depths of 4 and 8 cm, respectively. The saving effect of irrigation water was 28.5% than that of conventional water management treatments, and the amount of nitrogen runoff was decreased by 18.5% with intermittent irrigation. However, the phosphorus runoff was not different between the different water management treatments in paddy fields.