In a previous paper, ambient vibration tests were conducted on a cable stayed bridge with resilient-friction base isolation systems (R-FBI) to extract the dynamic characteristics of the bridge and compare the results with a seismic analysis model. In this paper, a nonlinear seismic analysis model was established for analysis of the bridge to compare the difference in seismic responses between nonlinear time history analysis and multi-mode spectral analysis methods in the seismic design phase of cable supported bridges. Through these studies, it was confirmed that the seismic design procedures of the “Korean Highway Bridge Design Code (Limit State Design) for Cable Supported Bridges” is not suitable for cable supported bridges installed with R-FBI. Therefore, to reflect the actual dynamic characteristics of the R-FBI installed on cable-supported bridges, an improved seismic design procedure is proposed that applies the seismic analysis method differently depending on the seismic isolation effect of the R-FBI for each seismic performance level.

In this study, a field bridge test was conducted to find the dynamic properties of cable supported bridges with resilient-friction base isolation systems (R-FBI). Various ambient vibration tests were performed to estimate dynamic properties of a test bridge using trucks in a non-transportation state before opening of the bridge and by ordinary traffic loadings about one year later after opening of the bridge. The dynamic properties found from the results of the tests were compared with an analysis model. From the result of the ambient vibration tests of the cable supported bridge with R-FBI, it was confirmed that the dynamic properties were sensitive to the stiffness of the R-FBI in the bridge, and the seismic analysis model of the test bridge using the effective stiffness of the R-FBI was insufficient for reflecting the dynamic behavior of the bridge. In the case of cable supported bridges, the seismic design must follow the “Korean Highway Bridge Design Code (Limit State Design) for Cable supported bridges.” Therefore, in order to reflect the actual behavior characteristics of the R-FBI installed on cable-supported bridges, an improved seismic design procedure should be proposed.

최근 한국에서 발생한 Salmonella로 인한 식중독 사고 는 2018년 9월 학교급식에서 제공된 초콜릿 무스 케이크가 원인이 되었다. 이 연구의 목적은 Salmonella Typhimurium이 인위적으로 접종된 무스케이크와 티라미수에서 3M Molecular Detection Assay 2 –Salmonella와 식품공전에 등재된 방법인 분리배지와 real-time PCR을 비교하는 것이었다. 무스케이크 2종과 티라미수 2종 25 g에 225 mL BPW를 넣고 37oC에서 24시간 동안 증균 배양하 였다. 배양 후, 3M Molecular Detection Assay 2 – Salmonella, 분리배지 그리고 real-time PCR로 분석하였다. 초콜릿 무스 케이크를 제외하고 3가지 방법은 유사한 결 과를 보였다. 초콜릿 무스 케이크에서 분리배지와 3M Molecular Detection Assay 2 –Salmonella는 모든 접종수 준에서 동일한 결과를 나타낸 반면 real-time PCR은 104 CFU/25g수준에서 1번의 양성결과를 제외하고 모두 검출 되지 않았다. 초콜릿 무스에 S. Typhimurium을 102 CFU/ 25 g 수준으로 접종하였을때, real-time PCR를 이용한 검출은 15%에서는 부분적인 음성을 나타냈고, 20-100% 함량의 초콜릿 무스에서는 모두 음성이었다. Real-time PCR 로는 chocolate이 15% 이상 함유된 식품에서의 Salmonella 균 검출이 불가능하였지만, LMAP 기반의 3M Molecular Detection Assay 2으로는 chocolate 농도에 관계없이 검출이 가능하였다.

Mycoplasma spp. are extracellular bacteria that colonize on the respiratory epithelium of humans and animals. It is a causative agent of pneumonia commonly complicated by opportunistic infectious bacteria. Mycoplasma spp. infection cause relatively mild disease in the absence of environmental stressors, but when complicated by secondary bacterial invaders the resultant disease can cause obvious clinical disease and severe production losses in intensively reared pigs Mycoplasma spp. are highly fastidious bacteria, difficult to culture and slow growing. Many species of Mycoplasma spp. are important pathogens causing respiratory infection in animals and known to induce huge economic losses. The aims of the present study were to develop a rapid isolation and culture method of wild type Mycoplasma spp. in pigs. We used Mycoplasma spp. genus specific direct PCR without DNA extraction procedure using PhireⓇ Animal Tissue Direct PCR Kit from the lung tissues with pneumonia lesions. Therefore, we could save the time for tissue processing and increase the accuracy of Mycoplasma spp. inclusion prediction in lung tissues. Thereafter, we used the optimized media to isolate and culture Mycoplasma spp. As the results, Mycoplasma spp. could be isolated and cultured quickly and efficiently. These results could provide an efficient strategy and method for the rapid and accurate isolation and culture of wild type Mycoplasma spp. in pigs.

The most effective microbial strains with the best ability to reduce complex odor were isolated from earthworm and activated sludge and identified using a 16S rDNA method. The isolated strains, Staphylococcus cohnii HYC-3 and S. carnosus JYC-4, were inoculated into the odor vials that had been left for 48 hours in water containing sesame dregs, and after 3 days, the ammonia was reduced to 5 ppm and 3 ppm from the initial 25 ppm, respectively. Complex odor was reduced to 2.5 and 2.2, respectively, while the control group maintained an odor of 5. The isolates were grown in the order of 30°C > 40°C > 20°C > 10°C. For HYC-3 and JYC-4, the optimum pH was 7 and 10, respectively, and the strains grew well at neutral pH ranges. To monitor the amount of microorganisms remaining in the environment by using the strain as a preparation for odor reduction, a probe for real-time PCR was designed. Through the quantitative and sensitivity tests on the developed strains, it was found that they showed excellent sensitivity.

본 논문에서는 비선형 지진격리교량의 최적 설계 방법을 제시하였다. 최적설계를 위한 목적함수로는 교각과 지진격리장치의 파괴확률을 고려하였으며, 상충하는 두 목적함수를 동시에 최적화하는 다수의 해를 효율적으로 검색하고자 유전자 알고리즘에 기반한 다목적 최적화기법을 도입하였다. 또한, 최적화 과정에서 요구되는 다수의 비선형 시간이력해석을 수행하지 않고도 교량의 확률적 응답을 효율적으로 예측할 수 있는 추계학적 선형화 방법을 접목하였다. 제시하는 방법의 효율성을 검증하기 위한 수치 예로서 실제 교량인 남한강교를 고려하였고, 제안하는 방법과 기존 비선형 시간이력해석을 이용한 생애주기비용 기반 설계법을 각각 적용하여 내진성능을 비교하였다. 내진성능을 비교한 결과, 제시하는 방법이 기존의 비용에 기반한 최적설계보다 우수한 성능 및 경제성을 보임을 검증하였다. 또한, 다양한 지진하중에 대해서도 제안된 방법이 보다 개선된 성능을 보임을 확인하였다.

면진격리 고무베어링의 설계법을 수정하여 구조물의 성능점 예측을 위한 간편한 해석방법을 제안하였다. 이러한 적용이 가능한 것은 구조물이 지진력의 작용으로 인하여 손상을 입게 되면 구조물의 항복 후 강성은 연화되고, 이로 말미암아 구조물의 동적 특성이 장주기화 하기 때문이다. 제안된 해석법이 기존의 방법에 비하여 우월한 것은 능력스펙트럼법이 요구하는대로 보유능력곡선과 요구량스펙트럼을 가속도-변위 좌표계로 치환하지 않고서도 비교적 정확한 성능점을 예측할 수 있다는 것이다. 제안된 방법의 타당성은 문헌에서 보이는 정확한 값과의 비교에 의하여 입증하였다.

In this paper, a fault detection method for operator failures using the observation technique is proposed. The suggested algorithm is extended using the conventional sensor/actuator fault detection method. First, it is assumed that operator failure affects human work operations, as it is an external input signal. With this assumption, a human work model with operator failure is suggested. Second, an unknown input observer with proportional and integral gains is introduced. The characteristic of this observer of estimating an external signal without an exact input is shown, and the conditions for the detection of an operator failure are proposed. Finally, by simulating the container crane operations, it is verified that the observer can accurately detect an operator failure and estimate its magnitude from the given internal signal.