The main objective of this research is to analyze the concept of gender difference through the language used by different genders shown on TV advertisements. Along with TV advertisements, this study further investigates on the cognition of gender differences through the questionnaire specifically discussing the language used by male and female students. In order to set the background, this research surveys the definitive characteristics of the language with respect to gender difference. Further, by examining the language used by different genders shown on all mass media including TV advertisements, there is an existence of gender differences in terms of linguistic expressions. Second, the field research on cognition was conducted by collecting the data of the middle and high school students. The result of the questionnaire reveals that male and female students choose their words without recognizing the gender differences in their oral or written expressions. As indicates, the cognition of language used by people reflects the explicit use of the language which plays a central role in linguistic change. This research concludes that the language used by male should include the characteristics of the female language in order to change the perception of gender difference.