In moth, pheromone biosynthesis activating neuropeptide (PBAN) regulates pheromone biosynthesis by binding to its receptor (PBANr). In this study, we cloned a PBANr gene (Mvi-PBANr) from sex pheromone gland in M. vitrata, which encodes 475 amino acid and includes 7 transmembrane domains. As a results of phylogenetic analysis, Mvi-PBANr is clustered with lepidopteran PBANrs. Mvi-PBANr was investigated for the effect of pheromone biosynthesis via RNA interference (RNAi), gas chromatography (GC) and bioassay. Consequently, expression level of Mvi-PBANr suppressed via RNAi, resulting in decrease pheromone component (E10E12-16:Ald). Mating rate was also reduced when performing the RNAi. These results revealed that Mvi-PBANr played important role in the pheromone biosynthesis in M. vitrata, and Mvi-PBANr can be used as new pest control targets.

갈색여치는 최근 대발생으로 과수농가에서 피해가 급격히 증가하고 있다. 갈색여치의 종령 약충과 미성숙 성충, 성숙 성충에 대한 교미행동 및 일일행동패턴에 대하여 조사하였다. 일일행동패턴은 휴식, 이동, 섭식을 반복하였다. 종령 약충과 미성숙 성충의 일일행동패턴은 유사했다. 종령 약충은 평균 10:06에 활동을 시작하고 21:12에 휴식을 취한다. 미성숙 성충은 평균 11:30에 활동을 시작하고 20:08에 휴식을 취한다. 그러나 성숙 성충의 경우 15:30에 활동을 시작하는 차이를 보였다. 성숙 성충은 특정시간(약 20:00)대에 교미행동을 보인다. 갈색여치의 교미행동은 암컷의 spermatophylax를 섭취하게 되는데 다 섭취하기까지 270-360분이 소요된다. 갈색여치의 행동패턴은 이들의 발생양상과 생태를 알 수 있어 갈색여치를 방제하기 위한 기초자료로 이용될 수 있을 것이다.

팥나방(Matsumuraeses phaseoli, (Matsumura))은 팥의 꽃과 꼬투리를 가해하는 해충이다. 본 연구에서는 팥나방 성충 우화와 교미 시간대, 성충 나이 및 더듬이 제거가 교미에 미치는 영향을 조사하였다. 성충 우화는 16L:8D 광 조건에서 불이 켜지고 4시간 이내에 대부분 이뤄졌다. 대부분의 교미가 암 기간 동안 이뤄졌으나, 불이 켜진 직후에도 교미하는 개체들이 일부 관찰되었다. 우화 당일의 성충은 교미를 하지 않았으나 우화 후 4일된 성충의 교미율이 가장 높았다. 더듬이가 제거된 수컷 또는 암컷은 더듬이가 제거되지 않은 반대 성의 정상 성충과 교미를 하지 못하였다.

In Hymenoptera, thanks to its haplodiploid sex determination, arrhenotokous parasitoid females are able to control offspring sex ratio by determining whether to use sperm or not at the time of oviposition. However, the offspring sex ratio may also be influenced by the mating behavior of a male. When a male searches females, he will have more chances to encounter with other males in a high male density population than low male density population. If the male is competitive, the male can find females faster and may have longer copulation time, and eventually transfer more sperm than other males. In this study, we investigated mate searching time, copulation duration and offspring sex ratio as a function of the male density. Although the results show difference between searching time and copulation duration, they are not statistically significant. We will discuss the statistical consequence, male density dependent offspring sex ratio and its evolutionary implications.

일부 고주파 음파 처리가 파밤나방(Spodoptera exigua)의 생리변화를 유발시킨다. 이 연구는 초음파( 20 kHz) 처리가 파밤나방 유충 섭식, 용 발육 및 성충 교미행동에 미치는 영향을 분석하였다. 초음파 처리는 5령충의 섭식 활동을 억제시켰다. 특별히 30 kHz 또는 45 kHz 초음파 처리를 받은 유충은 50% 이상의 섭식활동이 감소하였다. 이러한 초음파 처리를 받은 유충은 혈장의 주요 영양물질 함량이 변동되었다. 혈장 단백질은 처리 초음파의 주파수 증가에 따라 감소하였다. 그러나 혈당은 처리 초음파의 주파수 증가에 따라 증가하였다. 지질 함량은 30 kHz 처리까지는 증가하다가 이후 감소하였다. 파밤나방 5령의 혈구, 지방체 및 표피세포의 세 조직은 스트레스 관련 유전자들인 세 종류의 열충격단백질과 apolipophorin III를 발현시켰다. 그러나 초음파를 처리할 경우 일부 스트레스 관련 유전자들의 발현을 크게 억제시켰다. 초음파 처리는 또한 용발육을 억제시켜, 용기간을 연장시키고 성충으로 우화를 현격하게 낮추었다. 끝으로 초음파 처리는 성충의 교미행동을 억제시켜 암컷의 산란력을 뚜렷하게 낮추었다. 이러한 결과는 초음파가 파밤나방의 생리적 스트레스로 작용하고 있다는 것을 제시하고 있다.

Since its first discovery in Jinchoen in 1998, three types of Bemisia tabaci have been recorded in the Repulic of Korea. Because B. tabaci damages the high-return produce such as eggplant and bell pepper, non-chemical methods are sought to control the B. tabaci populations. We studied the mating behaviors of Q-type B. tabaci by video-recording 20 virgin pairs using digital microscopes, of which 10 had a successful mating. The mating behaviors of Q-type males can be divided into searching, paralleling to female, mounting and wing-flicking stages, whereas those of females were almost motionless throughout the mating sequence. During the paralleling stage, males also exhibited antennal drumming and undulation behavior. B. tabaci is know to use vibratory signals during mating. To understand the frequency and function of vibratory signals, we are simultaneously video-recording mating behaviors and measuring vibratory signals by means of a laser vibrometer.

Ascotis selenaria, one of the major Geometridae moths, annually damages the citrus leaves and fruits. This study was focused to identify the sex pheromone components of A. selenaria in order to monitoring and control its population. Adult female and male were usually emerged at the time between 2 hour before and 2 hour afterlights-off in 16L:8D photoperiod. Mating, irrespective in female age, started at 2 hour after lights-off and peaked between 4 hour and 6 hour after lights-off. They copulated for 3 hour 47 min per pair. Gland extract of virgin female was analyzed with GC-MS and GC-EAD.Z,Z-6,9-3,4 epoxy nonadecadiene (Z,Z-6,9-3,4 epo- 19Hy) was identified as the candidate. Both of stereo isomer, Z,Z-6,9-3R,4S epo- 19Hy and Z,Z-6,9-3S,4R epo- 19Hy, showed a strong EAG response in male antennae. Further study like field attraction test should be needed to clarify the sex pheromone composition and in A. selenaria. Age and time were related with the amount of sex pheromone components biosynthesized in A. selenaria gland. 0 day old female had 112.7ng per gland at 5 hour after lights off and the amount was decreased as they aged. The sex pheromone biosynthesis started from lights-off and showed the increasing tendency during scotophase.

A cDNA of PBAN receptor (Plx-PBANR) isolated from female pheromone gland of the diamondback moth (DBM, Plutella xylostella (L.) encodes 338 amino acids. Plx-PBANR includes 7 transmembranes, indicating it belongs to G-protein coupled receptor family. Plx-PBANR showed high similarities with other moth PBANRs and its expression was only found in female pheromone gland, demonstrating that pheromone gland is the only molecular target of Plx-PBAN. To accomplish the funcional expression of Plx-PBANR, Human uterus carcinoma was stably transfected with Plx-PBANR gene and Plx-PBANR expression was confirmed by RT-PCR analysis. Plx-PBANR expressing cells increased level of Ca2+ influx when challenged with Plx-PBAN and Hez-PBAN from Heliothis zea, as ionomycin as a positive control does. To inhibit Plx-PBNAR expression in vivo, RNAi fragment for Plx-PBANR was injected into pupae. Suppression of PBANR expression was confirmed by RT-PCR and also induced inhibition of mating behavior in adults, revealing that reproductive organ of the female has no spermatocyte and that there are no successful reproductive behaviors. RNAi-treated adults showed reduced pheromone production. These results suggests that inhibition of PBANR expression affects the molecular biological events of PBAN and eventually suppresses mating behavior.

Field collected larvae inside the pumpkin fruit in Jinan, Chonbuk Province were kept in the laboratory at 24±2℃, RH 65±5%, and 14L:10D until they pupated and eclosed. Adults were fed with dry-milk based diet. Mating behavior was observed when they were 40 days old in a field cage at dusk in July and October, 2008. Wing vibration in male took place under the light intensity of 200 lux-20 lux, however, most commonly under 30 lux. Mounting between male and female was observed under 150 lux-30 lux after the act of wing vibration, also most frequently under 30 lux. Copulation occurred under 150 lux-20 lux. Females approached face to face to males which had been engaged in wing vibration and the copulation was successful for up to 64%. Copulation never occurred when a female positioned in front or at side of the male that was not engaged in wing vibration.

Temperature is an important inorganic factor which affects on every aspect in life of phytoseiid mites in general including N. californicus in this study. Cumulative and short-term effects of temperature through different life stages (immature, mating and oviposition periods) on mating duration and total fecundity of N. californicus were investigated at temperatures of 18, 25, 30 and 350C with a photoperiod of 16L:8D. Throughout experiments, females mated once and egg production was observed until they stop oviposition.

멸구류의 난기생봉인 Anagrus incarnatus Haliday의 기주탐색, 고매행동, 산란습성 그리고 생색형태 조사한 결과는 다음과 같다. 난괴가 있는 엽초까지 도달하는데 소요되는 시간은 평균188.5초였고 기주에 도달후 안테나의 drumming시간은 17.5초, tapping 72.4초, 삽입하여 산란하는데 206.7초 걸렸으며 교미 시간은 16~21초 정도였다. 본 종은 양성생식과 단위생식을 겸하며 교미시의 암수 비율은 약 3:1이었고 자성단위 생식형이었다.

흰불나방을 재료로 곤충의 교미습성중 우화시간, 교미시간, 1회교미에 소요되는 시간 및 나방의 우화후일령이 교미율에 미치는 영향등을 밝히기 위하여 실내시험한 바 다음과 같은 결과를 얻었다. 1 흰불나방은 오후 3시부터 우화가 시작되어 오후 12시경에 끝나며 우화최성시각은 오후 7-8시 사이었다. 이때 암컷은 숫컷은 우화하였다. 2) 흰불나방은 오전 5-6시 사이에 교미하여 그 외 다른 시간에는 교미하지 않는다. 1회 교미시간은 최단이 7시간, 최장이 17시간이며 그 평균은 12시간이었다. 3) 흰불나방은 2회까지 교미할 수 있으며 1회 교미하는 것이 대부분이다. 실내시험결과 암컷은 1회 교미가, 2회 교미가 , 미교미가 , 이었으며 숫컷은 1회 교미가 , 2회 교미가 , 미교미가 이었다. 야외에서 채집된 암컷의 교미는 1회가 , 2회가 , 미교미가로서 야외의 것이 실내의 것에 비하여 2회교미하는 율이 높았다. 4) 흰불나방은 우화후 4일까지 교미한다. 우화된지 6-15시간후부터 교미하는데 이때에 의 높은 교미율을 보이지만 4일에는 의 낮은 교미율을 보인다. 5일 이후 교미하는 것을 관찰할 수 없었다. 자웅보다 동일 일령인 것 끼리 교미하며 일령이 다른 것과는 거의 교미하지 않는다.

This study has been conducted to investigate the mating behavior, time sequence of mating and time limit with which female can fulfill spawning and brooding after pre-spawning molting in the giant fresh-water prawn, macrobrachium rosenbergii reared in the laboratory. the results obtained were summarized as follows. Mating happened spontaneously as the following sequences: courting gesture, seizure of female by male, mounting, turning of female, copulation. The entire mating lated approximately seven minutes. The endopodite of 2nd pleopod of male bore the appendix masculins whcih are male's secondary sex characteristic. cinciulli was formed at the distal portion of appendix interna which are on the upper portion of appendix masculins and helped stabilizing the connection of each side of 2nd pleopod during mating. After the connection of each side of 2nd plepod was fixed on the posterior portion of the thoracic sternum in female, a gelatinous spermatophore that emitted at the basipodite of 5th pereiopod during courting gesture was deposited on the ventral groove of 2nd, 3rd, 4th and 5th pereiopod. time limit which female can fulfill copulation and brooding was from 3 hours to 15 hours after pre-spawning molting.