A mono-type glycerine fatty acid ester compound was chemically synthesized using medium-chain fatty acids with antibacterial activity, and the physicochemical quality and antibacterial activity of the synthesized glycerine medium-chain fatty acid ester compound were measured. At a reaction molar ratio of MCT(medium chain triglyceride) to glycerine of 1:2.5, 48.15% mono ester was produced. The physicochemical analysis of the synthesized glycerine fatty acid ester compound showed an acid content of 0.38~0.60%, which tended to increase slightly as the glycerine molar ratio increased. The saponification value decreased as the synthesized molar ratio of glycerine increased from 218 to 284. The number of bacteria was measured to confirm the degree of antibacterial activity of glycerine medium-chain fatty acid esters against food poisoning bacteria, Bacillus cereus and Salmonella typhi. The number of bacteria significantly decreased as the MCT:glycerine molar ratio increased. In particular, the antibacterial effect between the treatment groups was the highest when at an MCT:glycerine molar ratio of 1:2.5.

본 연구는 식물성 불포화지방산 복합제로 이루어진 보조사료를 첨가하여 이유자돈의 균일성과 증체성적에 미치는 효과를 구명하기 위하여 수행하였다. 공시동물은 일반 종돈장의 Landrace 모돈 110두와 포유자돈 248두를 공시하였다. 시험구는 모돈시험에서는 장쇄지방산 복합제제를 분만사입식부터 이유시까지 약 4주간 급여하였고 자돈시험에서는 액상 중쇄지방산 complex를 포유기간 중 2회 구강투여 하였다. 모돈 시험에서 복당생존자돈수와 복당생시체중은 처리구간 차이를 보이지 않았으나, 복당이유자돈수와 복당이유체중에서는 시험구가 대조구에 비해 유의적으로 높았다(P<0.01). 모돈의 사료섭취량은 시험구가 유의적으로 높았으며, 발정재귀일령은 유의적으로 차이는 없었으나 시험구에서 평균 0.3일 정도 빠르게 나타났다. 포유기간내 일당증체량 역시 이유체중과 양의 상관관계를 갖고 있어 유의적인 차이가 있는 것으로 나타났다. 자돈시험 결과 시험구는 포유기간내 이유체중이 대조구에 비해 유의적으로 높았으며 생시체중 대비 증체량도 같은 기간에 많은 차이를 나타내었다. 자돈의 이유체중에 대한 변이의 지표를 보면 시험구와 대조구간 변이의 차이는 없는 것으로 나타나 개체간의 균일성에 대해서는 처리간차이는 나타나지 않았다. 결론적으로 포유기의 장쇄지방산 급여는 모돈의 젖에서 올레인산, 리놀산 등불포화지방산의 함량을 증가시키고, 젖 분비량과 사료섭취량을 증가시켜 자돈의 초기증체에 기여하는 것으로 나타났고, 자돈에 대한 중쇄지방산의 급여도 이유체중을 증가시키는 효과를 가져왔다.

In resent year there has been research on benefits of conjugated linoleic acid (CLA) and docosahexaenoic acid (DHA) for human health. The effect of a fat supplement made from seaweeds meal plus palm olein meal (FS) and replacement of concentrate on milk production, and milk fatty acid (FA) profiles of dairy cows fed grass silage based diets was evaluated. Ten Holstein lactating cows were divided into a two groups. The cows in a group were fed concentrate at 40% on TDN basis (control). The cows in another group (Treatment) were supplemented 0.5㎏/day/cow of FS as a substitute for 2㎏/day/cow of concentrate. The medium chain fatty acids and DHA content of FS were 345 ㎎/g DM and 35 ㎎/g DM, respectively. Total DMI, DM digestibility and milk production as well as milk composition did not differ between the two groups. The intake of ether extract was higher (P＜0.01) in Treatment than control group (1.1 ㎏/day/cow vs. 1.3 ㎏/day/cow). The short and medium chain of FA in the milk did not differ between two groups. The trans11C18:1vaccenic acid, cis9trans11CLA and C18:3 concentrations in the milk were higher (P＜0.01) in Treatment than control group(1.88 g/100gFA vs. 1.03 g/100gFA, 0.93 g/100gFA vs. 0.51 g/100gFA and 0.48 g/100gFA vs. 0.48 g/100gFA). However, the C18:2 concentration in the milk was lower (P＜0.05) in Treatment than Control group (1.38 g/100gFA vs.1.17 g/100gFA). There were detected 0.13 g/100gFA of DHA in the milk in Treatment group. Results indicated that addition of the FS has the potential of increasing CLA and DHA in cow's milk.