Thus far, there have been many studies focusing on meat quality improvement for Hanwoo (Korean cattle). It is considered that studies on meat quality have been actively conducted because beef consumption has changed from quantity to quality since early 1990's. Moreover, many studies on meat quality using maker assisted selection (MAS) have been conducted. However, there is a need to study quantity of meat in order to enhance the economic effect by genetic diversity and quantity of meat for Hanwoo. Therefore, in order to use a myostatin gene affecting meat quantity among quantitative genes for MAS, this study analyzed the association between genetic variation of myostatin gene in individuals of Hanwoo and carcass traits. Analysis between genotypes and individual phenotypic characteristics was performed by ANOVA using ASREML. Association analysis between these 10 SNPs and phenotypic characteristics (carcass weight, eye muscle area, backfat thickness, marbling score, yield index), and there were significant differences in 5 SNPs. As a result, there were five significant differences. Although all the SNPs did not show significant differences in each trait in this study, some SNPs showed significant differences in each trait. The diversity of myostatin gene was found in Hanwoo.