This study was conducted to evaluate the effects of temperature and nematicides on endospore attachment characteristics of 2 Korean isolates of nematodeparasitic bacteria, Pasteuria penetrans. P. penetrans CJ-1 and 98-35 isolates and 3 species of root-knot nematodes in U. of Florida were used ; Meloidogyne incognita (MI), M. arenaria (MA), and M. javanica (MJ). Three nematicide levels of 2, 4, and 6 ppm were compared by treating Vydate and Nemacur in the endospore containing soils. The endospores were attached to the root-knot nematode juveniles by centrifugal method. Reproduction of the isolates was checked by observing root-knot nematode females harvested from inoculated tomato roots. 1. Among the two Korean isolates, 98-35 showed attachments to MI, MA, and MJ juveniles, but it could not reproduce in any species. CJ-1 reproduced on all the 3 tested root-knot nematode species. 2. Endospores pretreated in -14°C showed higher attachment rate than those pretreated in 4, 24, and 40℃. 3. Nemacur treatments significantly suppressed the attachment of the endospores to the juveniles, while Vydate treatment effects were negligible.