Gynura procumbens (Family Asteraceae) is a medicinal plant commonly found in tropical Asia countries such as China, Thailand, Indonesia, Malaysia, and Vietnam. Traditionally, it is widely used in many different countries for the treatment of a wide variety of health ailments such as kidney discomfort, rheumatism, diabetes mellitus, constipation, and hypertension. The objective of this study was to select the natural growth media for hydroponic cultivation of Gynura procumbens. The commercially available thirty four different organic nutrient solutions were analyzed for growing Gynura procumbens. After analysis, two organic nutrient solution were selected, mixed (pH 5.5~6.5 / EC 0.5~1.0 ds/m) and then used as organic fertilizers. Thirty day old seedlings were planted and the environmental conditions of the rhizosphere were also examined to select the natural media composition. The minimum temperature of rhizosphere by type of natural media was highest in cocopeat media and lowest in perlite media. Furthermore, plant growth was examined from 50 days old seedlings, and vermiculite was observed to be most effective and perlite was the least effective component as natural media. The total polyphenol content of the studied plant material was also higher in vermiculite in comparison to other growth media. The Gynura procumbens yields were also higher in vermiculite in comparison to other growth media. Thus, vermiculite can be used to improve Gynura procumbens plant growth as a natural growth media.