This study presented a development of a phototactic chamber used for pest monitoring. The chamber was constructed by opaque acrylic body. Transparent acrylic wall of the chamber for light-exposure were fitted at both side end parts of the inside chamber. Side parts of the outside chamber were made of removable cover in combination with the air circulation system and light source such as LED or fluorescent. The insect entrance holes was positioned at the center part of the chamber to efficiently dispersed pests, and then nylon net was equipped inside the chamber to prevent the escape of inserted pests. Two opaque partition walls of the inside chamber were made of the movable plate, in order to the control of the light-exposure and the response termination. We also carried out behavioral experiment against various pest species by using the phototactic chamber. Consequently, the phototactic chamber was confirmed suitable result of behavioral experiment. Therefore, we believed that the test chamber help to understand the phototactic responses of various pests.