급냉법에 의한 역 열유도상전이(RTIPS) 공정을 사용하여 mesoporous polystyrene (PS), polyethersulfone (PES) 막 을 제조하였다. 급냉법에 의한 RTIPS 공정은 급냉 및 승온 시 도포 용액 내 용매 분자들의 결정 생성 및 성장을 통해 나노 규 모의 상전이를 야기시켜 mesoporous 기공들을 형성된다. 시차주사열량계(TA: DSC) 사용해 측정된 사용 용매 dimethylformamide (DMF)와 여러 고분자 함량의 고분자용액들에 대한 엔탈피 변화와 주사현미경(SEM)을 사용하여 측정한 고분자함량에 따른 제조된 막 구조, 그리고 비표면적 분석기(BET) 사용하여 측정한 고분자 함량에 따라 제조된 막의 기공크기분포 및 표준편 차 분석을 통해 RTIPS 공정 시 상전이 거동을 살펴보았다.

Soybean lecithin liposomes composed phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol and phosphatidic acid were prepared by using the previously developed supercritical reverse phase evaporation method. The effect of phospholipid composition on the formation of liposomes and physicochemical properties were examined by means of trapping efficiency measurements, transmission electron microscopy, dynamic light scattering and zeta potential measurements. The trapping efficiency of liposomes for D-(+)-glucose made of CNA-Ⅰ which contains approximately 95% phosphatidyl choline is higher than that of CNA-II and CNA-O which contain approximately 32% phosphatidyl choline. However there is no any difference between the trapping efficiency of liposomes for D-(+)-glucose made of CNA-II which has saturated hydrocarbons tails and that of liposomes made of CNA-O which has unsaturated hydrocarbon chains. The electron micrographs of liposomes made of CNA-II and CNA-O show small spherical liposomes with diameter of 0.1~0.25μm, while that of CNA-I shows large unilamellar liposomes with diameter of 0.2~1.2μm. These results clearly show that phospholipid structure of phosphatidylcholine allows an efficient preparation of large unilamellar liposomes and a high trapping efficiency for water soluble substances. Liposomes made of CNA-II and CNA-O remained well-dispersed for at least 14 days, while liposome suspension made of CNA-I separated in two phase at 14 days due to aggregation and fusion of liposomes. The dispersibility of liposomes made of CNA-I is lower than that of CNA-II and CNA-O due to the smallar zeta potential of CNA-I.

The lipids from the seeds of Pinus koraiensis mostly composed of triacylglycerols (TGs), in which linoleic acid (46.2 mol%) and oleic acid (25.6 mol%) are present as main components in the fatty acid composition. Surprisingly, they also have unusual fatty acids with δ5-double bond systems such as δ5.9.12-C18:3 (16.0 mol%), δ5.9-C18:2 (2.3 mol%) and δ5.11.14-C20:3 (0.8 mol%). Saturated fatty acids of palmitic, stearic and arachidic acid were present in less than 8.0 mol%. TG was resolved into 17 fractions by reverse-phase HPLC according to so-called partition number (PN) suggested by Plattner, in which TG molecules with δ5-NMDB acyl chains eluted later than did those with δ9-MDB acyl radicals. Ag+-HPLC separated the TG into 14 fractions more clearly than did those with δ9-MDB acyl radicals. Ag+-HPLC separated the TG into 14 fractions more clearly than did reverse-phase HPLC, and the complexity of δ5.9.12-C18:3 moiety with silver ion impregnated in the column bed was in the level between δ9.12.15-C18:3 (C18:3Ω3) and C18:2Ω6 (δ9.12-C18:2). In the Ag+-HPLC, it was found that the molecular species having a given-numbered double bonds widely spreaded in the acyl chains eluted earlier than those concentrated in one acyl chain. The main molecular species are (C18:2Ω6)2/δ5.9.12-C18:3 (14.8 mol%), C18:1Ω9/C18:2Ω6)2 (12.8 mol%) and C18:1Ω9/C18:2Ω6/δ5.9.12-C18:3 (10.9 mol%).