This study investigated the effect of manufacturing variables (including heating temperature) on the physicochemical properties of nanoemulsion delivery system (NDS) prepared with WPI/Inulin Maillard conjugate and to study how the physicochemical properties of NDS affected the bioaccessibility of lycopene. The functional properties of the WPI/Inulin Maillard conjugate were determined using the OPA method, interfacial tension, and EAI. The physicochemical and morphological properties of NDS were measured using Zetasizer and TEM, respectively. The bioaccessibility of lycopene in the WPI/Inulin Maillard conjugate based NDS was measured using a spectrophotometer. As the pH and heating temperature increased, the Maillard conjugation efficiency increased significantly (p<0.0001). The emulsifying properties of the WPI/Inulin Maillard conjugate were greater than those of WPI. A WPI/Inulin Maillard conjugate based NDS with a size of ~180 nm was observed in TEM images while the droplet size of the WPI/Inulin Maillard conjugate based NDS was smaller than that of the WPI based NDS. During in vitro digestion, no significant changes in the droplet size and PDI of NDS were observed in the mouth and stomach phases, whereas in the intestinal phase, the droplet size and PDI increased significantly (p<0.0001). Moreover, the bioaccessibility of lycopene in the WPI/Inulin Maillard conjugate based NDS significantly increased (p<0.0001), compared with that of the WPI based NDS. There was a significant (p<0.05) increase in the bioaccessibility of lycopene with a decrease in the interfacial tension and droplet size of NDS. In conclusion, WPI/Inulin Maillard conjugate based NDS can be used to enhance the bioaccessibility of lycopene.

Five insecticides (Acrinathrin, Dinotefuran, Emamectin benzoate, Chlorfenapyr and fluxametamide) approved for tomato cultivation were evaluated in Frankliniella occidentalis populations collected from Chungcheong province (Cheongyang, Chungju and Gongju). Leaf dip bioassay was used to evaluate resistance levels (LC50). Bioassays on Acrinathrin demonstrated higher LC50 concentration in evaluated populations. In particular, the Chungju population was 745.61 times the recommended concentration of the insecticide. Other remarkable resistance levels were recorded for the Dinotefuran with 435.06 times and 196.29 times the recommended concentrations for the populations from Chungju and Gongju, respectively. Bioassays for Emamectin benzoate, Chlorfenapyr and Fluxametamide showed low resistance to insecticides in the evaluated populations.

Yellow flower thrips (WFT), Frankliniella occidentalis is mainly controlled using chemical control methods. But the continuous use of chemical pesticides in greenhouse may contribute to development of insecticide resistance. Therefore, in this study, we evaluated the insecticidal activity of eleven insecticides against the WFT occurring in greenhouse pepper cultivation in the Gyeonggi province. The results showed no resistance in treatments with emamectin benzoate, fluxametamide, and flometoquin while high levels of resistance were recorded in treatments with acrinathrin, acetamiprid, and dinotefuran. The Anseong and Yeoju population was more resistant against spinetoram and chlorfluazuron, respectively, than populations from other regions.

As the size of the gaming market continues to grow, game engines like Unity and Unreal are constantly being developed and updated for multi-platform support. In particular, Unity divides the Render Pipeline to be used. Among them, the Universal Render Pipeline (URP) has the advantage of supporting various platforms while improving performance by reducing draw calls and batches at the same quality settings and reducing the load on the CPU and GPU. However, only limited global illumination is supported in the real-time 3D rendering area within URP, such as Baked GI and Enlighten GI, and Enlighten GI will be deprecated after 2024LST, so real-time global illumination is not available in URP, which may reduce the realism and immersion of global illumination in games or applications that use URP. The main objective of this research is to incorporate precomputed spherical harmonics into URP to enable dynamic GI updates in real-time. The approach proposed in this research is to compute the coefficients of spherical harmonics offline and then run them within the URP rendering pipeline, where work exists to effectively simulate dynamic lighting conditions. To demonstrate this, experiments were conducted by changing the rotation and color of the lights, and a comparative analysis was performed to demonstrate the effectiveness of the theory proposed in this work.

Background: Although an understanding of the proliferation and differentiation of fish female germline stem cells (GSCs) is very important, an appropriate threedimensional (3D) research model to study them is not well established. As a part of the development of stable 3D culture system for fish female GSCs, we conducted this study to establish a 3D aggregate culture system of ovarian cells in marine medaka, Oryzias dancena. Methods: Ovarian cells were separated by Percoll density gradient centrifugation and two different cell populations were cultured in suspension to form ovarian cell aggregates to find suitable cell populations for its formation. Ovarian cell aggregates formed from different cell populations were evaluated by histology and gene expression analyses. To evaluate the media supplements, ovarian cell aggregate culture was performed under different media conditions, and the morphology, viability, size, gene expression, histology, and E2 secretion of ovarian cell aggregates were analyzed. Results: Ovarian cell aggregates were able to be formed well under specific culture conditions that used ultra-low attachment 96 well plate, complete mESM2, and the cell populations from top to 50% layers after separation of ovarian cells. Moreover, they were able to maintain minimal ovarian function such as germ cell maintenance and E2 synthesis for a short period. Conclusions: We established basic conditions for the culture of O. dancena ovarian cell aggregates. Additional efforts will be required to further optimize the culture conditions so that the ovarian cell aggregates can retain the improved ovarian functions for a longer period of time.

The secondary growth model for Salmonella was developed based on the artificial neural network (ANN) with data collected from ComBase and FoodData Central. In addition to the existing secondary model variables (temperature, pH, Na+, and water contents), more input variables (sugar, carbohydrate, lipid, and protein contents) were considered. The output variables were microbial growth parameters (lag phase duration [l] and maximum growth rate [mmax]). A commercial ANN program (NeuralWorks Predict) was utilized with training at 80%, validation at 10%, and test data at 10%. ANN models were created using all data and cleansed data. Using the cleansed data, the training/testing root mean square error (RMSE) for mmax improved from 0.14/0.16 to 0.11/0.14, whereas the RMSE for l was still not acceptable, from 11.94/33.03 to 7.09/4.18. The l data were divided into two ranges with high and low goodness of fit, whereas the ANN model for each field was built, resulting in an optimally low RMSE.

Amorphous In-Ga-Zn-O (a-IGZO) thin film transistors (TFTs) with a coplanar structure were fabricated to investigate the feasibility of their potential application in large size organic light emitting diodes (OLEDs). Drain currents, used as functions of the gate voltages for the TFTs, showed the output currents had slight differences in the saturation region, just as the output currents of the etch stopper TFTs did. The maximum difference in the threshold voltages of the In-Ga-Zn-O (a-IGZO) TFTs was as small as approximately 0.57 V. After the application of a positive bias voltage stress for 50,000 s, the values of the threshold voltage of the coplanar structure TFTs were only slightly shifted, by 0.18 V, indicative of their stability. The coplanar structure TFTs were embedded in OLEDs and exhibited a maximum luminance as large as 500 nits, and their color gamut satisfied 99 % of the digital cinema initiatives, confirming their suitability for large size and high resolution OLEDs. Further, the image density of large-size OLEDs embedded with the coplanar structure TFTs was significantly enhanced compared with OLEDs embedded with conventional TFTs.

Helina Robineau-Desvoidy (어리가시집파리속, 신칭)은 집파리과(Muscidae) 가시집파리아과(Phaoniinae)에 속하는 큰 분류군 중 하나 로 전세계에 540여종이 기록되어 있다. 그러나 한국에서는 아직까지 기록이 이루어진 바가 없으며, 이번 연구를 통해 한국미기록속 Helina Robineau-Desvoidy 및 미기록종 H. deleta (Stein, 1914), H. evecta (Harris, 1780), H. subpubiseta Xue, 1986을 처음으로 보고한다. 또한, 분류 학적 정보, 형태학적 사진 및 검색표를 작성하였다.

Brachydeutera Loew, 1862(코주부물가파리속, 신칭)는 물가파리과, 물가파리아과에 속하는 작은 분류군이다. 지금까지 이 속에는 전 세계 적으로 16종이 기록되어 있으며, 모든 동물구계에서 발견되지만 아열대 및 열대 지방, 특히 아프리카와 아시아에서 종 다양성이 풍부하다. 본 연구 를 통해 Brachydeutera속을 국내 미기록종 B. meridionalis (Rondani, 1856)(코주부물가파리, 신칭)과 함께 처음으로 보고하며, 이 종에 대한 진단 형질, 형태학적 사진 및 생태사진을 제공하였다.

자생 부추속 식물 중 강부추(Allium thunbergii for. rheophytum ined.)와 갯부추(A. pseudojaponicum Makino)는 관상용, 식 용 및 약용자원으로 가치가 있으나 육묘를 위한 생육환경조건 구명이 미비하여 연구할 필요성이 있다. 본 연구는 강부추와 갯부추의 육묘에 미치는 플러그 셀 크기, 차광률, 시비처리에 따른 영향을 구명하기 위하여 실험을 수행하였다. 강부추와 갯부추를 육묘한 결과, 플러그 셀 크기에서는 50, 72, 105, 128, 162, 200셀 처리 중 용적이 가장 큰 50셀에서 초장, 엽 수, 근수, 그리고 근장의 생육이 우수하였다. 그러나 생산비용 과 플러그 육묘의 효율성을 고려하여 105셀 이상의 플러그 트레이 중에서 선택하여 육묘하는 것이 효과적이라 판단된다. 차광률에 따른 유묘는 0, 30, 60, 90% 처리 중 30~60% 차 광처리에서 초장, 근수, 그리고 근장이 유의적으로 높게 측정 되어 생육이 양호하였다. 시비처리에서 생중량과 건중량을 제 외한 생육지표를 검토했을 때, 강부추의 적정 시비처리는 속 효성 고형비료(DO-PRO) 0.1g, 갯부추는 속효성 액체비료 (Peters) 주 1회 8mL 엽면시비처리였고 두 종 모두 속효성 시비처리가 효과적이었다. 강부추와 갯부추의 초기 생육에는 30~60% 차광처리가 된 재배플롯에서 원예상토가 충진된 128셀 플러그 트레이에 종자를 파종한후, DO-PRO 0.1g 또 는 Peters 8mL를 주 1회 엽면시비하면서 재배하는 것이 효 과적이라 판단된다.

Foot-and-mouth disease (FMD), which affects cloven-hoofed animals, is economically important because of its highly contagious nature. FMD virus (FMDV), the causative agent of FMD, involves seven serotypes (O, A, Asia1, C, and SAT 1-3). Serotype Asia1 is unique to the Asian territory and is subdivided into nine genetic groups (G-I-IX) based on nucleotide variations in the VP1 sequence. Asia1 Shamir, the most representative Asia1 vaccine, is not highly protective against the Asia1/MOG/05 (G-V) lineage found in North Korea in 2007. Therefore, we investigated whether a chimeric virus strain (Asia1/MOG/Shamir), in which the VP4, VP2, and VP3 sequences of Asia1/MOG/05 were combined with the VP1 sequence of Asia1 Shamir, can simultaneously protect against both viruses. We determined the optimal viral growth conditions for the commercial utilization of this chimeric virus strain. Of the three types of cell culture media, the Cellvento medium resulted in the highest amount of antigen in the samples. The chimeric strain was proliferated in a small bioreactor to produce a test vaccine, and its immunogenicity was evaluated in pigs. The virus neutralization (VN) titer against the Asia1 Shamir virus was > 1/100 after the second immunization with the chimeric vaccine in pigs. In addition, a single dose of the test vaccine resulted in a VN titer of > 1/100 against the Asia1/MOG/05 strain. Taken together, our chimeric vaccine strain provided sufficient protection against the Asia1/MOG/05 and Asia1 Shamir viruses, suggesting its potential as a novel vaccine for both these strains.

본 논문에서는 몬스터에게 붙잡히지 않고 키를 찾아서 연구소를 탈출하는 공포 방탈출 게임을 제안한다. 제 안하는 게임을 진행한30명의 게임 플레이 로그 데이터 분석 결과와 설문조사 결과를 바탕으로, 제안하는 게 임의 특징을 분석한 결과는 다음과 같다. 첫째, 제안하는 게임은 다양한 아이템, 액션, 탈출 경로를 제공한다. 제안하는 게임이 숨을 곳도 많고 다양한 상호작용을 제공한다고 설문에서4점 이상 주었다. 또한, map의 footprint를 분석한 결과, 플레이어는 다양한 경로를 통해 키를 찾아서 탈출하였다. 둘째, 제안하는 게임은 외 관으로 기능을 추론할 수 있는 직관적인 오브젝트를 제공한다. 따라서, 플레이어는 시각적 공간 및 게임 아 이템 용도를 쉽게 파악하여 조작할 수 있다. 설문조사 결과에서, 플레이어는 조작감 관련 항목의 점수를4점 이상을 주었다. 셋째, 제안하는 게임에서 플레이어는 아이템이 충분할 때보다는 부족할 때 더 몰입을 잘 한 다. 게임 플레이 로그 데이터 분석 결과와 설문조사 결과에 따르면, 플레이어는 아이템이 부족할 때 더 크게 공포를 느끼고 상황에 몰입하여, 더 적극적으로 행동하게 되고 더 민감하게 반응한다.

This study aimed to prepare kombucha, a fermented tea beverage, containing Dendropanax morbiferus (DM) leaves and roots, and analyze its antioxidant and intracellular activities. We compared the pH change, total acidity, radical scavenging activity, and oxygen radical absorbance capacity (ORAC) of kombucha fermented with black tea alone and that with added DM leaves or roots during fermentation. Using RAW 264.7, we evaluated the effects of kombucha containing different DM parts on nitric oxide (NO) production and inflammation-related cytokine content in cells. Kombucha containing ethanol extracts of DM leaves (BTK-E-DML) and roots (BTK-E-DMR) showed higher radical scavenging activity and ORAC 3 d after fermentation than that prepared from black tea alone (BTK-Ori). In an in vitro experiment using RAW 264.7, samples were treated with 8 mg/mL kombucha considering cytotoxicity; the lipopolysaccharide (LPS)-induced NO content significantly reduced after BTK-E-DML and BTK-EDMR treatments compared with that after BTK-Ori treatment. Additionally, the levels of interleukin-6 and tumor necrosis factor-alpha, which were LPS-stimulated inflammatory cytokines, significantly decreased in cells treated with BTK-E-DML and BTK-E-DMR 15 d after fermentation compared with those treated with BTK-Ori. In conclusion, these results demonstrate that kombucha fermented with the leaves and roots of DM increases antioxidant activity and can significantly regulate inflammatory responses at the cellular level.