Pansies butterflies belong to the genus Junonia are widely distributed throughout Myanmar and 6 species of Junonia have been reported in Myanmar. Molecular comparison of the cytochrome c oxidase subunit I (COI) nucleotide sequences among the species of Junonia was conducted. The nucleotides divergence of COI sequences between species were 2.9 to 7.9% and sequences identity was 92.5-97.7%. The phylogenetic trees were constructed by using Neighbour-joining (NJ) and Maximum likelihood (ML) methods. In both methods, phylogentic trees were showed identical and were revealed that all sequences of Junonia species from Myanmar were in the same cluster with those of the same species from other countries.