Clover cyst nematode (Heterodera trifolii, CCN) is one of the important plantparasitic nematodes in the Republic of Korea, causing serious damage to Kimchi-cabbage in Gangwon province since 2017. Soil fumigants are the preferred choice for managing CCN. However, cabbage fields in Gangwon province have a slope, making it challenging to apply soil fumigants and use plastic mulch. Consequently, alternative materials should be developed for managing CCN. Nematicidal cover crops and resistant cultivars are among the alternatives. We conducted resistance screening of 82 white mustard (Sinapis alba) resources from a genebank at RDA against CCN for use as nematicidal cover crops or breeding materials. In the first assay (1st, 2nd, and 3rd pot experiments), 15 white mustard resources were selected, while others were susceptible to CCN. To confirm the reproducibility of resistance to CCN for 13 selected resources, the second assay was performed. As a result, five white mustard resource (IT297309, IT297312, IT302951, IT302953, IT302954) demonstrated resistance to CCN. These findings indicate the potential use of these five white mustard resources as nematicidal cover crops or for breeding cultivars of Kimchi-cabbage in Republic of Korea.

Myrosinases (thioglucosidases) catalyze the hydrolysis of a class of compounds called glucosinolates, of which the aglycones show various biological functions. It is often necessary to minimize the loss of myrosinase activity during thermal processing of cruciferous vegetables. Myrosinase was isolated from a popular spice, white mustard (Sinapis alba), and its thermal inactivation kinetics was investigated. The enzyme was extracted from white mustard seeds and purified by a sequential processes of ammonium sulfate fractionation, Concanavalin A-Sepharose column chromatography, and gel permeation chromatography. At least three isozymes were revealed by Concanavalin A-Sepharose column chromatography. The purity of the major myrosinase was examined by native polyacrylamide gel electrophoresis and on-gel activity staining with methyl red. The molecular weight of the major enzyme was estimated to be 171 kDa. When the consecutive step model was used for the thermal inactivation of the major myrosinase, its inactivation energy was 44.388 kJ/mol for the early stage of destruction and 32.019 kJ/mol for the late stage of destruction. When the distinct two enzymes model was used, the inactivation energy was 77.772 kJ/mol for the labile enzyme and 95.145 kJ/mol for the stable enzyme. The thermal inactivation energies lie within energy range causing nutrient destruction on heating.

사탕무씨스트선충의 밀도 감소를 위한 유인작물의 이용 가능성을 구명하기 위하여 white mustard(품종:Braco)와 메밀(품종: 약선메밀, 양절메밀)에 대한 뿌리침출액의 씨스트 선충 알부화 유인 효과와 선충감염 토양에서의 알 밀도 감소 효과를 각각 실내 검정과 온실에서 포트 검정하였다. 유인작물 뿌리침출액의 씨스트 알부화 유인 효과는 씨스트를 접종하고 6주후에 무처리 대비 white mutard 뿌리침출액은 2.6배, 약선메밀은 2.3배, 양절 메밀은 1.8배 씨스트 알에서 부화된 유충수가 증가하였다. 사탕무씨스트선충 감염 토양에 유인작물 재배 8주후에 white mustard는 81.0%, 양절메일은 72.6% 알 밀도가 감소하였으며, 씨스트선충은 증식되지 않았다. 사탕무씨스트선충 피해 경감을 위해 white mustard와 메밀의 활용 가치가 높을 것으로 사료된다.