무측지성 수분수용 사과 품종 개발을 위해 ‘홍로’를 자방친, ‘메이폴’을 화분친으로 하여 교배한 교배실생을 조사·선발하여 ‘팅커벨’로 명명, 국립종자원에 품종보호 등록을 완료하였다. ‘팅커벨’ 육성 과정은 2003년에 인공교배를 실시하여 2009년까지 과실 및 생육특성을 조사하여 1차 선발하였다. 계통명 ‘03-2-095’로 2010년부터 2012년까지 과일특성 및 주요 품종과의 교배친화성을 평가한 후 최종 선발하였다. ‘팅커벨’은 대조품종 ‘메이폴’에 비해 만개기가 약 2일 정도 늦고, 수세가 약하고 가지 발생이 거의 없는 무측지성을 보인다. 대조품종 ‘메이폴’의 과형이 ‘타원형’인데 비해 ‘팅커벨’의 과형은 ‘넓은 타원형’이다. ‘팅커벨’은 117g으로 ‘메이폴’보다 과실이 크고, 과피가 매끈하며 과분이 없다는 점에서 차이가 있었다. ‘팅커벨’의 당도는 12.3oBx로 ‘메이폴’ 10.7oBx 보다 높고, 산도는 1.2%로 ‘메이폴’ 1.8% 보다 낮아서 당산비가 ‘메이폴’ 보다 개선되었다. 주요 품종 과의 인공교배시 착과율은 ‘후지’ 90.8%, ‘홍로’ 94.9%, ‘쓰가루’ 71.0%로 매우 높을 뿐만 아니라 수폭은 70cm이므로 사과 밀식재배체계에 아주 적합한 수분수용 신품종으로 판단된다.

‘Mantari’ is a new variety of oyster mushroom for bottle culture. It was bred by crossingmonokaryons isolated from ‘DM11732’ and ‘Chunchu-2ho’. The optimum temperature for the mycelial growth was 26~29°C on PDA medium, and that for the primordia formation and the growth of fruiting body of ‘Mantari’ on sawdust mediawas 18°C and 16°C. It took 32 days to finish spawn running, 4 days to finish primordia formation, and 3 days to finish fruiting body growth in the bottle culture. The fruitcharacteristics: the pileus was round and gray-black in color, and the stipe was long, thin, and light gray in color. The yield per bottle was 179 g/900 ml and was 5% higher than that of the control variety (Chunchu-2ho). The physical properties of the fruit body were as follows: springiness, cohesive, gumminess, and brittleness of the stipe tissue were 96%, 76%, 160 g, and 15 kg, respectively.

Drawing on psychological reactance theory, this study examines consumers’ reactions to grocery categories’ variety reductions. Results show negative main effects on post-reduction satisfaction with variety and store patronage intentions, which are moderated by grocery category nature and by consumers’ intrinsic need for variety and attitude toward private label brands.

Since the first Korean button mushroom ‘Sae-Ah’ developed in 2010, most farmers had wanted to cultivate a new variety. A new variety, ‘SaeJeong’, was developed in 2011, but this variety was difficult to cultivate in mushroom farm. To solve the variety problem, a new variety ‘Sae-do’ was developed in 2012. The variety, ‘Sad-do’, was made by crossing hetrokaryon A175 and homokaryon ASI1346-15 selected by genetic analysis. The heterokaryon A175 is crossed line between ASI1038-211 and ASI1346-20. The mycelium of ‘Saedo’ on CDA (compost dextrose agar) grew well at 25°C. The optimum pin-heading temperature of it was 13-15°C and optimum growing temperature was 13-20°C. Earliness (days required from casing to first harvesting) was 2 days shorter than control ‘Saejeong’. Even though the diameter of mature cap and the length of stipe were shorter than a control, the individual weight of fruiting body was heavier because of thick pileus and stipe. Therefore, the yield was increased by 1.89 times than control and the hardness of pileus was stronger. The field experiment of ‘Saedo’ variety has been started since 2013. Most farmers evaluated that the variety has good compost colonization and cap shape, and high yield in harvesting. For that reason, ‘Saedo’ has occupied half of the of distribution rate of Korean varieties. Also distribution rate of Korean varieties was increased from 26.1% in 2014 to 37% in 2015.

‘Mantari’ is a new variety of oyster mushroom for the bottle culture. It was bred by mating with monokaryons isolated from ‘DM11732’ and ‘Chunchu-2ho’. The optimum temperature for the mycelial growth was 26~29°C on PDA medium and that for the primordia formation and the growth of fruiting body of ‘Mantari’ was 18°C and 16°C on sawdust media. It took 32 days to finish spawn running, 4 days to finish primordia formation, 3 days to finish fruitbody growth in the bottle culture. In the characteristics of fruit body, pilei were round type and dark grayish, stipe color was gray-white color and stipe shape was long and thin. The yield per bottle was 179g/900ml and was 5% higher than that of control veriety(Chunchu-2ho). As results of the physical properties of fruit body, springness, cohesive, gumminess and brittleness of stipe tissue were 96%, 76%, 160g and 15kg, respectively.

The table grape 'Hongisul' with strong cold and disease resistance has high sugar, low acidity and excellent palatability. However, this grape has a problem of poor coloring. This study was carried out to improve the fruit color through leaf removal treatments in ‘Hongisul’ grapes. The leaf removal treatment involved removing 2 leaves and 4 leaves around the grape cluster at the start of veraison. The light exposure value of clusters increased 4 leaves removal clusters compared with those of the 2 leaves removal and non-treatment. However, there was no difference in the ambient temperature among the three treatments. The improved light exposure caused by leaf removal increased soluble solids content and the ratios of hunter a value (red) in the grape skins, while total acidity had decreased more in the leaf removal. These findings are expected to improve viticultural practices with the aim of producing red-colored grape.

This study investigated the changes in chemical components, antioxidant compounds, and activity before (BG) and after germinated (AG) brown rice in order to promote the availability of these beneficial factors. The GABA content of BG and AG brown rice were 0.07~6.61 and 11.13~49.72 mg/100 g, respectively. The α-amylase activity of AG brown rice was 1.77~70.25 unit/g fold higher than it was in BG brown rice. Total polyphenol and flavonoid contents, which are known to be related to antioxidation, were higher in BG brown rice than in AG brown rice. The total polyphenol contents of BG and AG brown rice were 10.52~36.38 and 11.38~26.33 mg/100 g, and the total flavonoid contents were 3.55~13.39 and 3.52~9.78 mg/100 g, respectively. Also, DPPH radical scavenging activity was 57.64~251.34 and 50.49~213.35 mg TE/100 g, respectively. ABTS radical scavenging activity and total tannin content showed a similar trend to DPPH radical scavenging activity. We expect that this data will be useful in the manufacturing of food products.

Although expression of foreign genes in a crop species has been target techniques for powerful protection against insect pests, classical breeding programs using varietal resistance of a crop are still being processed. It is, however, frequently difficult to find key products expressed from resistance-related genes in the variety, and those action mechanism. Here, an unterminated story about seed of a mungbean (Vigna radiata) variety with high resistance against the adzuki bean weevil and the bean bug is introduced. The two insects cannot survive on seeds of the variety, although they well develop on susceptible ones. Molecular markers linked to the resistance were selected through BAC library screening and near isogenic lines, and finally a bruchid resistance gene was suggested after map-based cloning approaches. Starvation and chemicals were suggested for a resistant mechanism and a related factor through feeding and behavior experiments, respectively. The seed flour was extracted with organic solvents, and isolated into several fractions on chromatography. Several peaks on HPLC in a fraction were related with the high mortality of the bean bug. A partial structure in an isolated chemical was observed before full identification.

‘Lucky one’ is a new orchardgrass (Dactylis glomerata L.) variety developed by the National Institute of Animal Science (NIAS) in 2014. Four superior clones were selected and polycrossed for seed production to develope this new variety of orchardgrass. The agronomic growth characteristics and forage productivity of ‘Lucky one’ were examined at Cheonan from 2010 to 2011. Regional adaption trials were conducted in Cheonan, Hoengseong, Jinju, and Jeju from 2012 to 2014. ‘Lucky one’ showed growth habit of medium type in fall and spring. It has short flag leaf length with long upper internode. Plant height of ‘Lucky one’ was 3 cm more than that of control variety, ‘Amba’. Its heading date was 2 days later than that of control variety ‘Amba’. Characteristics such as waterlogging and disease resistance of ‘Lucky one’ were stronger or better than those of control variety, ‘Amba’, Especially, dry matter yield of ‘Lucky one’ (16,191 kg/ha) was increased 38% compared to that of control variety, ‘Amba’(11,778 kg/ha). Nutritive feed values of ‘Lucky one’ appeared to be similar to those of control variety ‘Amba’ except in Vitro dry matter digestibility (IVDMD) and crude protein content.

‘Onnuri 2ho’ is a new orchardgrass (Dactylis glomerata L.) variety developed by the National Institute of Animal Science (NIAS) in 2014. Four superior clones were selected and polycrossed for seed production to develop this new variety of orchardgrass. The agronomic growth characteristics and forage productivity of ‘Onnuri 2ho’ were examined at Cheonan from 2010 to 2011. Regional adaptation trials were conducted in Cheonan, Hoengseong, Jinju and Jeju from 2012 to 2014. ‘Onnuri 2ho’ showed growth habit of medium type in fall and spring. It is long in length of flag leaf with medium upper internode. Plant height of ‘Onnuri 2ho’ was 4 cm more than that of control variety, ‘Amba’. Its heading date was 4 days later than compared to control variety, ‘Amba’. Characteristics such as waterlogging and disease resistance of ‘Onnuri 2ho’ were stronger or better than those of control variety, ‘Amba’, Especially, the dry matter yield of ‘Onnuri 2ho’ (15,814 kg/ha) was increased 34 % compared to that of control variety, Amba (11,778 kg/ha). Nutritive feed values of ‘Onnuri 2ho’ appeared to be similar to that of control variety ‘Amba’ except in Vitro dry matter digestibility (IVDMD).

단간 내도복성 ‘소담찰’ 수수의 논 재배 시 안정적인 수량확보를 위해 파종시기 및 재식거리에 따른 생육 특성과 종실생산성에 대한 시험을 2014년 경기도 수원의 국립식량과학원시험포장에서 수행한 결과를 요약하면 다음과 같다. 1. 출수기는 6월 10일 파종에서 62일로 가장 빨랐으며, 비닐피복재배에서 파종기가 늦을수록 출수기가 빨라지는 경향이뚜렷하였다. 2. 파종기에 따라서는 6월 20일 파종에서 생육이 급격히 감소하였고 간경, 분지수, 천립중에서 유의적인 차이를 보였고,피복유무에 따른 간장, 간경, 분지수에서도 유의성을 보였다. 3. 주당종실중과 종실수량은 파종기별 비닐피복재배에서는유의적인 차이가 인정되지 않았으나 무피복재배에서는 6월 20일 파종에서 주당종실중이 17.7g, 종실수량이 212 kg/10a으로가장 적었으며, 파종기 간에 유의적인 차이를 보였다. 4. 소담찰 수수는 간장이 평균 90 cm로 매우 작아 논 재배에서 문제가 되는 도복에 강한 경향을 보였으며, 이삭길이는소식재배에서 길어지는 경향을 보였고 간장, 간경, 분지수, 이삭길이, 천립중은 재식밀도 간에 유의성을 보이지 않았다. 5. 주당종실중과 종실수량은 재식밀도 간에 유의한 영향을주지 않았으나 주당종실중은 60×25cm (6,600본/10a)의 소식재배에서 48.0 g으로 가장 많았으며, 10a당 종실수량은 60 × 15 cm(1본)과 60 × 30 cm(2본)의 밀식재배에서 각각 427 kg, 415 kg으로 가장 증수되어 소담찰은 밀식적응성이 높은 경향이었다. 6. 따라서 소담찰 수수는 기계수확 등 생력기술을 적용하여논 대면적 재배를 위해 파종기는 6월 10일, 재식밀도는11,000주/10 a [60 × 15 cm(1본), 60 × 30 cm (2본)가 유리한 것으로 판단된다.

농업환경의 급속한 변화에 의한 농업생명공학과 GM작물의증가는 인간의 건강과 자연환경에 대한 깊은 관심을 초래하였고, 이에 대한 환경위험 여부를 확인하기 위해 GM작물의 안전성 평가가 필수 요소가 되었다. 이에 본 연구는 가뭄저항성(Agb0103)벼와 일미벼의 잉어(Carp, Cyprinus carpio)와 미꾸리(Loach, Misgurnus anguillicaudatus)에 대한 급성독성시험을 평가한 결과 48시간과 96시간의 LC50은 1,000 mg/L이상으로 분석되었다. 48시간과 96시간의 무영향농도는 1,000 mg/L이었다. 잉어와 미꾸리의 급성독성 시험기간 중 가뭄저항성(Agb0103)벼와 일미벼 간의 체중, 전장, 수온, DO 및 pH에대한 유의적인 차이는 없었다.

The spotted-wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), originally distributed across a few Asian countries, including South Korea, has invaded North America and Europe, but is absent from Australia. In order to export the South Korean grape cultivar Campbell Early to Australia, its potential to serve as oviposition and development medium for SWD must first be determined. In this study, we determined the oviposition and development potential of SWD on Campbell Early, after elucidating the SWD life cycle and establishing an artificial diet-based mass-culturing system. An investigation of the life cycle under five temperature regimes (16, 19, 22, 25, and 28°C) showed that the durations of the egg, larval, and adult stages were shorten when temperature was increased from 16, 19, 22, 25, and 28°C, but pupal duration was shortest at 25°C and extended again at 28°C. A test of oviposition and development potential of SWD on Campbell Early grape clusters showed oviposition of 30.8 ± 6.8 eggs per cluster of injured grapes and 157.7 ± 16.2 eggs on a culture dish of artificial diet. However, in a similar experiment using uninjured grape clusters, only a single egg was deposited on the grape skin, which soon dried. In light of these results, newly harvested grapes left at vineyards during daily harvests are unlikely to serve as an oviposition and development medium for SWD, as long as the grapes remain uninjured.

‘Nongjin-go’ is a new breed strain of Lentinula edodes, saw-dust bag variety. It is a cross combination of dikaryonLentinula edodes ASI 3305mut and monokaryon L5-16 of L. edodes ASI 3305(Sanjo701ho). We crossbred them by 2011 andverified productive capacity from 2012 to 2013 in Rural Development Administration. Optimum temperature of mycelial growthis 30oC and it of fruit-body primodium formation is range from 15 to 23oC. Nongjigo is agricultured at mid-high temperaturewell. Fruiting body is platy-hemisphere, light brown and centralizing. And bast is formed around edge of pileus. Yield productionsper period is regular than ‘Sanjo701’. Plastic bag culture medium is 1.5 kilogram and culture periods are 90~100 days. As itsbrowning of pileus in culture is a little slow, Light and ventilation is needed a lot in light-culturing. Humidity is controlled properlyfor its color in fruit-body growing. Tested culture medium is consisted of 80% Oak-Tree saw-dust and 20% rice-bran.

‘Baekjung’ adaptable to high temperature was made by crossing between monokaryon derived from selfing of brownstrain and monokaryon derived from Korea white strain. In the condition that temperature is maintained at 10oC without lowtemperature of 4oC suppressing treatment and wrapping during cultivation period, it showed good productivity thanUri1ho(control). The optimum temperature of mycelial growth was 30oC and that of fruiting body initiation and developmentwere 14oC and 7oC, respectively. The days for the fruiting and yield were 7days and 277±11.2g per 1,100ml bottle,respectively. This variety needed high concentration of carbon dioxide up to 4,000 ppm for the good quality.

느타리버섯(Pleurotus ostreatus)의 품종 육성을 위해서는 많은 노력과 시간을 필요로 하고 있어, 최근에는 분자육종방법으로 마커를 이용하여 빠르고 정확하게 교잡주를 선발하고 있다. 본 실험에서는 육종효율을 증진하기 위해 교잡효율이 높은 Di-Mon 교배방법으로 부친의 핵과 모친의 미토콘드리아를 가진 세포질전환 교잡주를 선발하였다. 느타리버섯 수한3호와 흑변이체의 교잡종인 ‘다굴’의 이핵균주에 춘추2호의 단핵균주와 교잡하여 120여개의 교잡주를 얻고 여기에 1차로 미토콘드리아 microsatellite DNA 마커 (MtPO1)를 이용하여 단핵균주 춘추2호의 미토콘드리아 밴드를 형성하는 교잡주 80개를 1차 선발하였다. 선발된 80균주중에서 URP 프라이머를 이용하여 ‘다굴’의 2핵균주 핵 DNA 패턴을 가진 26교잡주를 선발하였다. 이 중에서 수량성이 우수한 3개 교잡주의 자실체 특성을 조사한 결과 초발이일수가 2일이나 빠르면서 수량성이 좋은 품종을 선발하여 ‘천화심’이라 명명하였다. 천화심은 수량이 높으면서 여름에도 재배가 가능해 춘추2호의 약점을 보완함으로써 춘추대체품종으로도 재배가 가능하리라 본다.

팽이버섯 신품종 ‘흑향’은 ‘갈뫼’와 수집 야생균주인 ‘CBMFV-10’으로부터 분리한 단포자를 교배함으로써 육성된 품종으로, 갓 색이 진한 갈색을 나타내며, 재배기간이 짧고, 식미감이 매우 우수하다. 병재배 시 배양 기간은 23일, 초발이 소요일수는 6일, 생육일수는 17일로 총재배기간이 46일이 소요되어 대조 품종 ‘갈뫼’에 비해 8일이 단축되었다. 갓 직경과 두께가 갈뫼에 비해 크거나 두꺼웠고, 갓 색은 진한 갈색을 나타냈다. 수량은 850 ml병 당 135 g으로 갈뫼 150 g 대비 10% 감소하였다. 하지만, 야생 팽이버섯처럼 식미감이 매우 우수하여, 차별화된 색과 맛으로 백색 팽이버섯 틈새시장 공략에 유리할 것으로 판단된다.

팽이버섯 신품종 ‘금향’은 ‘갈뫼’와 수집 야생균주인 ‘CBMFV-33’으로부터 분리한 단포자를 교배함으로써 육성된 품종으로, 갓 색이 진한 미색을 나타내며, 재배기간이 짧다. 병재배 시 배양 기간은 23일, 초발이 소요일수는 7일, 생육일수는 17일로 총재배기간이 47일이 소요되어 대조 품종 ‘갈뫼’에 비해 7일이 단축되었다. 갓 직경은 ‘갈뫼’에 비해 크고, 갓 색은 진한 미색을 나타냈다. 수량은 850ml 병 당 162 g으로 ‘갈뫼’ 대비 8% 증수되었다. 또한 야생 팽이버섯처럼 식미감도 우수하여, 차별화된 색과 맛으로 백색 팽이버섯의 틈새시장 공략에 유리할 것으로 판단된다.