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pp.113-117
본 연구는 임신 및 비임신 비장유래 macrophge의 체외배양에 의한 배양액내의 호르몬 생산을 조사하여, macrophge의 임신 관련 기전을 구명하기 위하여 실시하였으며, 임신 및 비임신된 도축 암소의 비장을 채취, macrophages를 분리채취하여 24~120시간동안 39에서 체외배양을 실시하였다. Progesterone의 생산은 24-96시간 동안에는 임신한 것이비임신에 비하여 생산율이 높았으며, 120시간에는 임신이 비임신에 비하여 유의적으로
This study was performed to investigate the hormonal changes in cultured medium during in vitro culture of bovine splenic macrophages. Both pregnant and non-pregnant slaughterhouse spleen were obtained and the macrophages were separated and cultured for 24~120 hrs at 39. Progesterone productions of pregnant group were higher than non-pregnant group for 24~96hrs and significantly higher for 120 hrs. The production of estradiol was higher in 24 hrs in pregnant group and no differences post 24 hrs. Insulin-like growth factor- I(IGF-I) production of pregnant was higher than non-pregnant group at all the culture time point. Inconclusion, splenic macrophages were able to produce peptides by in vitro culture and have important role for the successful pregnancy in bovine.
