Childhood obesity causes a higher risk of obesity, premature death and disability in adulthood. In addition, obese children experience an increased risk of respiratory problems, hypertension, cardiovascular disease, insulin resistance and psychological effects. This study aimed to investigate how an exercise intervention affects health-related physical fitness and inflammatory-related blood factors in obese children after. We hypothesized that there would be positive effects on serum levels of insulin-like growth factor-1 (IGF-1), connecting peptide(C-peptide) and resistin, as well as in muscle and cardiovascular-related physical capacities, after an exercise intervention in obese children. Thirty-seven obese children haveperformed health-related fitness tests and provided blood samples for the analysis of changes in circulating biomarkers, both before and after an 8-week exercise intervention, which includes stretching, aerobic exercise, resistance exercise and sports games. The results indicate that exercise training beneficially affects body compositions, especially percentage body fat and muscle mass, without influencing to body weight and height. The results of the physical fitness tests show that muscle and cardiovascular capacity were increased in obese children in response to exercise training. Simultaneously, the exercise training decreased circulating levels of C-peptide, which equated to a “large” effect size. Although there were no significant effects on the levels of IGF-1 and resistin, they show a “small” effect size. Therefore, our findings suggest that the exercise intervention have beneficial effects on body composition and physical fitness levels in obese children, whichmight be associated with the decline in circulating C-peptide.

The purpose of this study was to investigate the effect of rope skipping and band exercise on GH, IGF-1 and metabolic syndrome risk factors in female obese middle school students. Twenty female obese middle school students(%BF > 30%) were randomly assigned to a exercise group(EX, n=10) or control group(CON, n=10). The exercise group has performed rope skipping and band exercise for 12 weeks, 3time per week. Exercise intensity was increased gradually, from 40% to 70% of Heart Rate Reserve(HRR) and Rating of Perceived Exertion(RPE) 11-15. GH, IGF-1 and metabolic syndrome rist factors were GH, IGF-1 and metabolic syndrome risk factors were measured pre- and post-exercise program including rope skipping and band exercise. Levels of GH and IGF-1 were significantly increased (p<.01 and p<0.001, respectively) in exercise group after 12 weeks of exercise training. WC and TG were significantly decreased(p<.01) in exercise group after 12 weeks of training. HDL-C was significantly increased(p<.05) in exercise group after 12 weeks of training. In conclusion, rope skipping and band exercise were effective exercise program in improving GH, IGF-1 and metabolic syndrome risk factors in female obese middle school students.

Recently, with the increase of meat production, high quality and safety of meat have been strongly emphasized by Korean consumers. Marbling in beef has been regarded as an important criterion deciding meat quality in Korea. The purpose of this study was to identify the transcriptional level of insulin-like growth factor-1 (IGF-1) in longissimus muscle samples of 46 Hanwoo. The level of IGF-1 transcripts was measured by real-time polymerase chain reaction (PCR) and molecular connection of IGF-1 was analyzed using the Pathway Studio program (Ver 9.0). Increase of marbling score (MS) induced increase of IGF-1 transcripts level in the muscle and there is a significant correlation (p<0.05) between IGF-1 mRNA expression and MS. The pathway study showed that IGF-1 genes are regulated in insulin, fatty acid synthase, leptin, and corticotrophin releasing hormone. These results suggest that IGF-1 might be used as a useful marker for the improvement of economic traits in Hanwoo.

Chicken Insulin-like Growth Factor-1 (cIGF-1), one of the most important hormone for regulating physiological function includes body growth, muscle volume, bone density, chicken cell development and metabolism. In order to find in vitro Knokdown expression of cIGF-1, this study introduced tetracycline inducible RNA interference expression system (TetRNAi system). Tet system can inductively control high expression of extrinsic genes and expression of intrinsic genes. So it has advantages such as minimized physiological side-effects any cell and low cytotoxicity. RNAi system is proving to be a powerful experimental tool for inhibition of gene expression and post-transcriptional mechanism of gene silencing. RNAi is mediated by small interfering RNA (siRNA) consisting of 19- to 23- nucleotide double-stranded RNA duplexes that promote specific endonucleolytic cleavage of mRNA targets through an RNA-induced silencing. Then, this study RNAi-based gene knockdown can be achieved by retroviral-based expression systems. Stable integration of our inducible siRNA vector allowed the production of siRNA on doxycycline induction, followed by specific down regulation of chicken IGF-1 gene. Analyses of Real-time PCR to determine expression of the cIGF-1 gene showed successful from chicken embronic fibroblast (CEF) cells with the reduced rate of an approximately 92%. Our results demonstrate the successful regulation of cIGF-1 knockdown expression in CEF cells and support the application of an tetracycline inducible RNAi expression system in transgenic Mini chicken production. This research was supported by Bio-industry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

To study the signaling effect of insulin-like growth factor-Ⅰ(IGF-1), transgenic mice containing IGF-1 Receptor (IGF-1R) cDNA fused to metallothionein promoter were produced by DNA microinjection into the pronucleus of mouse zygote. Three founders were produced with transgenic mice containing IGF-1R gene. Transgenic mice lines contained approximately 4～20 copies of transgenes per cell and transmission of this gene into the progeny with Mendelian manner were determined. The founder mice were mated with normal mice to produce F1 mice and then F2 mice. Transmission rates of IGF-1R transgene in the progeny mice were 25～60% in F1 generation and 40～50% in F2 generation. The mRNA expression of IGF-1R transgene in liver was analyzed using RT-PCR for IGF-1R gene in liver. When body weights of transgenic pups were measured during 4, 10 and 14 weeks after birth, IGF-1R transgenic mice grew faster than non transgenic littermates. This study indicated that growth regulation by IGF-1 signaling through IGF-1R can be elucidated using IGF-1R transgenic mice.

다양한 종류의 생약재들이 동물과 인간의 성장률을 증진시키는 활성을 가지고 있는 것으로 보고되고 있다. 선정된 식물의 성장증진 효과를 확인하기 위해 실험동물로서 물고기와 돼지를 이용하였다. 물고기 실험에서 오가피 추출물과인진쑥 추출물이 가장 효과적인 것으로 나타났다 그리고 이들 오가피와 인진쑥 추출물을 돼지에 먹여본 결과 평균일일 증체율이 대조군에 비해 7.06%증가하였다. 이 결과를 검증하기위해 혈액 내 분비된 성장호르몬(GH)과 Insulin-like growth factor-1(IGF-1)의 양을 측정하였다. 오가피와 인진쑥 추출물을 먹인 돼지는 대조군에 비해 혈액 내로 분비된 GH와 IGF-1의 양이 훨씬 더 많았다. 인간에서 성장증진 효과를 알아보기 위해 본 연구자들은 오가피와 인진쑥 추출물을 혼합하여 쌀에 코팅한 키우미^(TM)(Kiwoomi^(TM))를 제조하였다. 키우미^(TM)를 초등학생들에게 먹였을 때 키 성장에 효과적인 것을 확인하였다. 키우미^(TH)를 먹인 아이들이 먹이지 않은 아이들보다 더 많이 성장하였다(약 2.14배). 결과적으로 이 기능성 쌀(Kiwoomi^(TM))이 다른 부작용 없이 아이들의 성장에 도움을 주는 것으로 판단된다.

MT-GHR(Growth hormone receptor)와 MT-IGF-IR(IGF-1 receptor)유전자를 구축하고 micromani-pulator를 이용하여 토끼 수정란에 유전자를 주입하여 형질전환토끼를 생산하였다. 본 연구에서의 형질전환토끼의 생산효율은 약 3%를 나타내었고 Growth Hormone receptor(GHR)를 가진 형질전환 토끼와 IGF-1 receptor(IGF-lR)를 가진 형질전환토끼를 10마리 이상씩 생산하였다. 또한 정상 토끼와 교배시켜 F₁ 새끼를 얻어 유전자가 다음세대에도 전달되는 것을 확인하였다. GHR 이나 IGF-1R 형질전환토끼의 성장률은 정상토끼보다는 약15∼25% 정도 빠른 경향을 나타냈고 특히 GHR 형질전환토끼의 성장률이 더 높은 것으로 드러나 GHR 및 IGF-lR유전자가 형질전환토끼에서 성장에 영향을 주었다는 것을 확인할 수 있었다.

본 연구는 돼지 체외수정란의 배양시스템의 최적화를 위하여 수행되었다. 기본배양액으로는 NC-SU-23을 사용하였으며, 실험 1에서는 IGF-I이 각각 0, 1, 10, 100 ng/의 농도로 첨가된 배양액에서 배양하여 분할율, 배반포 발달율과총 세포수를 확인하였다. 농도 경사에 따른 분할율은 차이가 없었으나(65.5%~69.5%), 배반포 발달율에 있어서는 10 ng/의 농도로 첨가하였을 경우가 가장 높았다(P<0.05). 실험 2는 배 아 유전자 활성

본 연구는 성장인자 EGF, IGF-1, EGF+IGF-1 이 소 난포란의 체외성숙에 미치는 영향을 규명하기 위하여 실시하였다. 소 난포란의 체외성숙 시 EGF를 농도별 (10, 50 및 100ng/m1)로 첨가하여 실험한 결과 통계학적 유의성은 인정되지 않았으나 24시간 배양 후 성숙율은 대조군에 비하여 높은 경향을 나타내었다. 소난포란의 체외성숙 시 EGF와 IGF-1을 병용 처리하여 실험한 결과, EGF 또는 IGF-1 단독처리 군에 비하여 병용처

Background : Insulin-like growth factor 1 (IGF-1) appears to enhance the differentiation of osteoblasts and to activate the mineralization of bone. Hence, the aim of this study was to investigate the effects of ginseng complex on the remodeling of rats tibia. Methods and Results : Ginseng complex significantly increased serum IGF-1 by 58% and 34.5% than the control, respectively. Treatment with α-amylase when manufacturing these extracts remarkably increased the concentration of IGF-1 by 63% and 36% above the control, respectively. This ways that this ginseng complex, especially α-amylase treated extracts, contained a higher level of IGF-1 secretion in the ginseng complex groups. In addition, increases of 8% in femuf length were found after 12 weeks of oral administration with ginseng complex (300mg/kg). Conclusion : These results mean that ginseng complex have beneficial effects on bone effects on bone growth via IGF-1.

인슐린 유사 성장 호르몬 1과 2 (IGF-1 & IGF-2)는 착상 전 초기배아 발생을 조절하는 중요한 요소이다. 생쥐 착상 전 초기배아에서 IGF-1의 역할에 관한 연구를 위해, IGF-1과 IGF-1 수용체의 전사물의 존재 여부를 난자와 착상 전 초기배아에서 조사하였다. 새로이 고안된 IGF-1 primer를 이용하여 난자에서 전사물을 검출하였다. 그리고, PCR 산물을 제한효소인 Msp I으로 절단하여 확인하였다. 이 실험에서 IGF-1과 IGF