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DNPH 유도체화 HPLC 방법을 이용한 카보닐 화합물 분석시 이동상 조성에 따른 알데하이드류의 분리 KCI 등재

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한국냄새환경학회지 (Journal of Korean Society of Odor Research and Engineering)
한국냄새환경학회 (Korean Society Of Odor Research And Engineering)
초록

An effective method, use of a 2,4-dinitrophenylhydrazine (DNPH) derivatization and high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, has been used for separation carbonyl compounds. In particular, acetonitrile/water composition as a mobile phase could not separated acrolein/acetone and 2-butanone/ butyraldehyde. In this study, however, that"s compounds separated using acetonitrile/water/tetrahydrofuran as a mobile phase. The resolution was 1.48 and 1.46, respectively. Also, aldehydes and ketones was shown different decrease rate and increase resolution at increase tetrahydrofuran ratio. But butyraldehyde become coeluted methacrolein.
Therefore, it is need to consider of mobile phase for separation of butyraldehyde.

목차
ABSTRACT
요약문
1. 서론
2. 실험방법
3. 결과 및 토의
4. 결론
감사의 글
참고문헌
저자
  • 유영재
  • 고진태
  • 김만구