The aim of this study is to develop the one dot rapid detection kit that meets the limit of detection (LOD) of KFDA (10 μg/kg) for Aflatoxin B1 (AFB1). The fabrication process of the one dot rapid detection kit, such as gold nanoparticle and AFB1-BSA conjugation, and AFB1-polyclonal antibody concentration was optimized. Fabricated rapid detection kits were tested with the standard AFB1 solutions and the solutions extracted from contaminated maize samples. The LOD in the standard AFB1 solution and extracted AFB1 solution was 5 μg/kg for both. This result is lower than the LOD of KFDA in food (10 μg/kg) and satisfies the LOD of EU (2-12 μg/kg) and Japan (10 μg/kg) in food.

• 최선미(한양대학교 식품영양학과) | Sunmi Choi
• 김기영(농촌진흥청 국립농업과학원 농업) | Giyoung Kim Corresponding author
• 이상대(농촌진흥청 국립농업과학원 농업공학부) | Sangdae Lee
• 문지혜(농촌진흥청 국립농업과학원 농업공학부) | Jihea Moon
• 엄애선(Department of Food and Nutrition, Hanyang University) | Aeson Om