느타리버섯은 가장 중요한 식용버섯 중 하나이다. 느타리버섯은 Pseudomonas tolaasii에 의한 세균성 갈변병에 매우 감수성이므로, 저항성 품종을 만들기 위한 노력의 하나로 누에에서 분리된 항 세균성 단백질인 누에신을 느타리버섯에서 과발현시키고자 하였다. 누에신 cDNA는 여름 느타리버섯의 β-tubulin 프로모터에 결합되어 pTRura3-2 vector와 함께 우라실 영양요구성 돌연변이 균주에 형질전환되었다. 누에신 cDNA가 형질전환된 느타리버섯을 genomic PCR과 Southern blot을 통하여 분리할 수가 있었으며, 이들 중 3개의 형질전환체가 누에신 유전자를 발현시킴을 확인하였다. 그러나 이들 형질전환체들에서 누에신 단백질을 검출할 수 없었으며, 또한 항 세균 효과도 확인할 수 없었다. 이들 결과는 형질전환기술을 이용한 병 저항성 개발 가능성을 보여주고 있다.

Pleurotus ostreatus, the oyster mushroom, is one of the most important edible mushrooms. It is especially susceptible to bacterial blotch disease, which is caused by Pseudomonas tolaasii. In order to develop bacterial blotch disease-resistant transgenic mushroom, NUECIN cDNA, a gene for an antibacterial peptide cloned from Bombyx mori, was overexpressed in Pleurotus ostreatus. NUECIN cDNA was fused to the β-TUBULIN promoter of oyster mushroom and co-transformed with the pTRura3-2 vector into the uracil auxotrophic mutant strain. Twelve transformants containing the Nuecin gene were identified by genomic PCR and Southern blot analysis. NUECIN gene expression was confirmed by Northern blot analysis. Three transformants showed the transcriptional expression of the gene. However, we could not detect expression of the protein in the transformants. This study showed the possibility of transgenic mushroom development for disease resistance.

ABSTRACT
 Introduction
 Materials and methods
  Strains and plasmids
  Vector constructs
  Transformation
  Genomic DNA isolation and Southern blot analysis
  Northern blot analysis of transformants
  Western blot analysis of transformants
 Results
  Isolation of transformants co-transformed withpTubnuecin and pTRura3-2
  NUECIN cDNA was transcriptionally expressed intransformants
  Transformants did not show antibacterial activityagainst P. tolaasii
  NUECIN protein was not detected in the transformants
 Discussion
 적요
 References

• Beom-Gi Kim(Division of Bio-crop development, Department of Agricultural Bitotechnology, National Academy of Agricultural Science, RDA)
• Eun-Young Yun( Division of Applied Entomology, Department of Agricultural Biology, National Academy of Agricultural Science, RDA) | Eun-Young Yun
• Youngbok Yoo( mushroom research division, National Institute of Horticultural and Herbal Science, RDA) | Youngbok Yoo