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        검색결과 196

        1.
        2024.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In this study, we investigated the microbial community of oyster mushrooms at different growth stages at the species level. Gram-positive bacteria were predominant in the presterilized medium. On the other hand, Gram-negative bacteria were predominant in the culture-completed medium, post-harvest medium, and fruiting bodies. In addition, Pseudomonas tolaasii, which is known to cause disease in mushrooms, was confirmed in the cultured medium, post-harvest medium, and fruiting bodies, and it was determined that the mycelium culture stage was contaminated, and the reason why no disease occurred was Sphingobacterium psychroaquaticum. It was confirmed that this was because the growth of Pseudomonas tolaasii was suppressed by producing a component called tolacin. As a result of confirming the diversity of microorganisms, it was confirmed that the presterilization medium contains a variety of microorganisms compared to other growth stages, and the diversity decreases in the order of culture completion medium, fruiting body, and post-harvest medium. showed a trend. As a result of microbial similarity analysis, it was confirmed that the cultured medium and the post-harvest medium showed similar microbial communities, and in the case of fruiting bodies, there were some similarities but overall differences.
        4,000원
        2.
        2024.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study analyzed the effects of different nitrogen sources in substrate composition on the growth of Pleurotus ostreatus, as well as the subsequent changes in flavor and antioxidant activity. The T2, composed of poplar sawdust, beet pulp, cotton seed dregs, and cotton seed coat in a ratio of 40:20:20:20, exhibited the highest yield at 156.6 g. The total polyphenol content and ABTS and DPPH radical scavenging activities were 8.25 mg GAE/g, 70%, and 49%, respectively, showing higher radical scavenging activity compared to the Control and T1. Additionally, varying nitrogen content resulted in distinct aroma patterns and is presumed to influence taste profiles such as sourness, umami, and saltiness.
        4,000원
        5.
        2023.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In this study, Pleurotus ostreatus No.42 was cultured in glucose-peptone-yeast-wheat bran medium using a previously reported novel rotary draft tube bioreactor. Versatile peroxidase (VP), a lignin-degrading enzyme, was isolated from a pellet-type mycelium culture grown in the medium for seven days. The VP was purified by sequentially applying ultra-filtration, DEAESepharose CL-6B column, and Mono Q column. SDS-PAGE analysis revealed the molecular weight of VP to be 36.4 KDa with an isoelectric point of 3.65. The amino acid sequence was confirmed as VTCATGQTT. The purified VP was observed to possess the property of not only oxidizing Mn ions but also decomposing veratryl alcohol, a non-phenolic compound. The catalytic ability of VP is a subject for future research.
        4,000원
        10.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The development of automated bottle cultivation systems has facilitated the large-scale production of Pleurotus ostreatus, a commonly cultivated oyster mushroom species in South Korea. However, as the consumption of this product is decreasing and production quantities are exceeding demand, farmers are seeking various other mushroom types and cultivars. In response to this, we have developed a new oyster mushroom cultivar named 'Sena'. This high-yielding cultivar has a white pileus and excellent quality. The white oyster mushroom cultivars 'Goni' and 'Miso' were selected as parental strains from the genetic resources of the National Institute of Horticultural and Herbal Science’s Mushroom Division. By crossing their monokaryons, hybrids were developed and subjected to cultivation trials and characteristic evaluations to select the superior cultivar. The optimal temperature for 'Sena' mycelial growth is 25–30°C, with inhibition occurring at temperatures above 30°C, whereas the temperature for mushroom growth is 14–18°C. The mushrooms grow in clusters, with the white pileus having a shallow funnel shape. Optimal mycelial growth occurs in malt extract agar medium. When cultivated in 1,100 cc bottles, the 'Sena' cultivar had 35 available individuals, surpassing the number 16 available from the control cultivar 'Goni'. The yield per bottle also increased by approximately 157 g, a 24% increase over the control cultivar amount. When 300 g samples of harvested mushrooms were packed and stored at 4°C in a cold storage facility for 28 days, the weight loss rate of ‘Sena’ was approximately 4.22%, lower than that of 'Goni'. Moreover, the changes in pileus and stipe whiteness (measuring 6.99 and 8.33, respectively) were also lower than those of the control cultivar. Since the appearance of a white cap is crucial for quality assessment, the 'Sena' cultivar is superior to the 'Goni' cultivar in terms of both weight and quality after undergoing lowtemperature storage.
        4,000원
        11.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        We conducted an on-site application study at the livestock cooperative fertilizer plant to compare the composting period, temperature change, moisture content, and chemical properties between livestock manure compost using sawdust as a moisture regulator with those using spent oyster mushroom substrate. The composting period, moisture content, and fertilizer composition of compost containing spent oyster mushroom substrate did not differ from that of conventional compost mixed with sawdust after the first and second fermentation and post-maturation stages, it was suitable as a material for manufacturing livestock manure compost. The spent oyster mushroom substrate also lower the production cost of livestock manure compost by replacing the more expensive sawdust. The developed technology is expected to contribute towards the utilization of by-products of the oyster mushroom harvest while simultaneously producing high quality livestock manure compost.
        4,000원
        12.
        2023.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The oyster mushroom is a widely cultivated edible mushroom in Korea. It is a regionally specialized crop in Gyeonggi-do, where 68% of the national production is produced. The present study was conducted to develop a new, highquality cultivar with stable cultivation. ‘Manseon’ is a new variety of oyster mushroom suitable for bottle culture. It was produced by mating monokaryons isolated from ‘HB-18’ and ‘P15159-16’. The optimal temperature for ‘Manseon’ mycelial growth was 26–29 °C on potato dextrose agar (PDA) medium and for primordia formation and the growth of fruit bodies the optimal temperature was 18–19 °C on sawdust medium. Spawn running required 30 d, primordia formation required 4 d, and fruit body growth in the bottle culture required 4 d. Regarding the characteristics of the fruit bodies, the pilei were round and dark grayish brown, stipe color was white, and stipe shape was short and thick. The yield per bottle was 192.7 g/1,100 cc, which was 9% higher than that of the control strain (Suhan-1ho). The physical properties of springiness, cohesiveness, gumminess, and brittleness of stipe tissue were 87.9%, 77.4%, 445.1 kgf, and 389.6 kgf, respectively.
        4,000원
        13.
        2023.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In this study, we aimed to compare the mycelial growth of Pleurotus ostreatus after medium supplementation with various amino acids at different concentrations to select the optimal medium nutrient composition for mycelial growth. The mycelial growth of P. ostreatus was investigated after adding four amino acids (tryptophan, threonine, methionine, and lysine) at 0.5% or 1% to the medium.The rate of P. ostreatus mycelial growthwas faster in the potato dextrose agar (PDA) medium supplemented with threonine at 0.5% or 1% than that of the control, whereas it was inhibited by tryptophan treatment. Supplementation of sawdust mediumwith all amino acids, except tryptophan, at 0.5% did not alter the mycelial growth, compared to the controls. However, addition of any amino acid to sawdust medium at a higher concentration (1%) inhibited the mycelial growth. The laccase acitivity of P. ostreatus mycelium cultured in PDA medium was the highest when threonine was added, and the lowest when tryptophane was added, consistent with the results of the mycelial growth. Therefore, the addition of threonine, methionine, or lysine to PDA medium at a concentration of 0.5-1%was effective for increasing the mycelial growth of P. ostreatus; however, it inhibited mycelial growth insawdust medium, suggesting that the effects of amino acids dependedon the medium nutrient composition.
        4,000원
        20.
        2022.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was conducted to investigate the growth and antioxidant activity of Pleurotus ostreatus mycelia grown in medium supplemented with Aronia. The diameter and dry weight of the mycelia were increased in the medium supplemented with Aronia compared with the untreated medium. The total polyphenol content of mycelia grown in medium supplemented with Aronia and untreated medium was 6.54 mg GAE/g and 5.77 mg GAE/g, respectively. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of mycelia grown in medium supplemented with Aronia and untreated medium was 61.81% and 49.65%, respectively. Moreover, the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity of mycelia grown in medium supplemented with Aronia and untreated medium was 59.83% and 52.66%, respectively. These results confirmed that P. ostreatus mycelial growth and antioxidant activity were increased when Aronia was added to the culture medium.
        4,000원
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