Satellite cells were derived from muscular tissue in postnatal pig. Satellite cell is an important to growth and development in animal tissues or organs. However, the progress underlying induced differentiation is not clear. The aim of this study was to evaluate the morphologic and the transcriptome changes in porcine satellite cell (PSC) treated with insulin, rosiglitazone, or dexamethasone respectively. PSC was obtained from postnatal muscle tissue. In study 1, for study the effect of insulin and FBS on the differentiated satellite cells, cells were cultured at absence or presence of insulin treated with FBS. Total RNA was extracted for determining the expression levels of myo-genic PAX3, PAX7, Myf5, MyoD, and myogenin genes by real-time PCR. Myogenic genes decreased expression levels of mRNA in treated with insulin. In study 2, in order to clarify the relationship between rosiglitazone and lipid in differentiated satellite cells, we further examined the effect of FBS on lipid accumulation in the presence or absence of the rosiglitazone and lipid. Significant differences were observed between rosiglitazone and lipid by FBS. The mRNA of FABP4 and PPARγ increased in rosiglitazone treatment. In study 3, we examined the effect of dexame-thasone on osteogenic differentiation in PSC. The mRNA was increased osteoblasotgenic ALP and ON genes treated with dexamethasone in 2% FBS. Dexamethasone induces osteoblastogenesis in differentiated PSC. Taken together, in differentiated PSCs, FABP4 and PPARγ increased to rosiglitazone. Whereas, no differences to FBS and lipid. These results were not comparable with previous reports. Our results suggest that adipogenic, myogenic, and osteoblasto-genic could be isolated from porcine skeletal muscle, and identify culture conditions which optimize proliferation and differentiation formation of PSC.

• Jin Young Jeong(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Jang Mi Kim(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Ramanna Valmiki Rajesh(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Sekar Suresh(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Gul Won Jang(Division of Planning and Coordination, National Institute of Animal Science, Rural Development Administration)
• Kyung-Tai Lee(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Tae Hun Kim(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Mina Park(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Hak Jae Jeong(Division of Animal Biotechnology, National Institute of Animal Science, ural Development Administration)
• Kyung Woon Kim(Division of Animal Biotechnology, National Institute of Animal Science, ural Development Administration)
• Yong Min Cho(Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration)
• Hyun-Jeong Lee(Division of Planning and Coordination, National Institute of Animal Science, Rural Development Administration) Corresponding author