논문 상세보기
pp.276-281
탈지미강으로부터 피틴산을 추출 및 정제하기 위한 최적조건을 설정하였다. 탈지미강을 10배수의 0.5% HCl 용액으로 1시간 처리하였을 때 피틴산의 추출효율이 가장 좋았으며 중화제로는 0.5% NaOH 용액이 적합하였다. 피틴산의 정제를 위하여 Diaion HP20 흡착 컬럼을 이용하여 불순물을 제거한 후 여러 가지 이온교환수지를 이용하여 피틴산을 정제하였을 때 Amberlite IRA-416 이온교환수지의 회수율이 가장 높았다. Amberlite IRA-416 이온교환수지를 이용하여 피틴산을 수지에 흡착시킨 다음 0.5% NaOH 용액으로 용출시켰을 경우 89%의 회수율을 나타내었다. 정제된 피틴산의 총단백질 함량은 0.14%(w/w)로 대부분의 단백질이 제거됨을 확인하였다.
The optimum condition for the extraction and purification processes of phytic acid from defatted rice bran was examined. The phytic acid was efficiently extracted when the defatted rice bran was treated with 10 volumes of 0.5% HCl for 1 hr. For the neutralization of acid-treated extract, 0.5% NaOH was the most acceptable. To purify phytic acid, Diaion HP20 resin was used to remove impurities from the extract. The flow-through was then loaded onto ion exchange columns packed with various resins and among them, Amberlite IRA-416 resin showed highest recovery yield. When the phytic acid was absorbed onto Amberlite IRA-416 resin and then eluted with 0.5% NaOH, 89% of applied phytic acid was eluted. Most proteins were removed from the purified phytic acid and total protein content of the phytic acid was 0.14% (w/w).
