SPR biosensors which belong to a family of thin film refractometry-based sensors measure refractive index changes produced by biomolecular interactions occurring at the surface of the sensors. The main advantage of SPR biosensors is to detect molecular interactions directly without the use of labels. This feature makes them possible to observe biomolecular interactions in real-time or near real-time. The non-specific binding between ligand and target analyte may, however, produce a false refractive index change resulting in false sensor response. The applications of SPR biosensors have involved biomolecular interaction kinetics analysis, affinity measurement, screening and concentration assay, and so on.

• 조용진(한국식품개발연구원) | Yong-Jin Cho Corresponding author