This study was carried out to provide accurate identification of Noctuidae, using the DNA barcode. In total, 2,040 adult specimens of 443 species were collected from 13 forest areas in Korea. In this study, we conducted the correct identification using external morphology and genitalic characters. In addition, we observed and compared the voucher specimens, preserved in the major entomological collections in Korea, including Hannam university Natural History Museum (102 species), Korea Forest Research Institute (8 species) and the National Science Museum (27 species) for correct identification. For extracting DNA sequences of the mitochondrial gene COI, the hind legs were detached and sampled with tweezers, providing about 25 mg of tissue sample. We amplified and sequenced the standard DNA barcode fragment of 648 basepairs. As a result, we have complete DNA barcodes for all 331 species. These results will be presented in Forest Pests Information Data Sheets for Noctuidae, containing DNA Barcode information, external morphology, ecological characteristics and phenology.