Field surveys for the B. tabaci species complex were conducted from 2009 to 2013 in Korea, and the results were compared with published data of the B. tabaci complex. Three species, Mediterranean, Middle East-Asia Minor 1, and JpL, were determined from several regions based on mitochondrial COI sequences. The Mediterranean was mainly collected in greenhouses, displacing the earlier invasive species, Middle East-Asia Minor, and the JpL was collected in the field. The JpL is newly confirmed as a unique species of B. tabaci complex in Korea and Japan.