We isolated two baculoviruses, Spodoptera litura granulovirus (SlGV) and S. litura nucleopolyhedrovirus (SlNPV) in the dead larvae of S. litura. The granule of SlGV were ovoidal shape with an approximate measure of 240-340 nm×140-180 nm, and each granule contained one single rod-shape virion with a mean size of 180-200 nm×20-40 nm. Whereas, the polyhedra of SlNPV were irregular in shape with a approximate diameter of 1.0-1.5 ㎛, and numerous virions comprised of the multinucleocapsid were contained in each polyhedra. The major component of occlusion bodies produced by SlGV and SlNPV were about 29 and 30 kDa, respectively. When the phylogenic relationship between these viruses were analyzed using the nucleotide sequences of granulin gene from SlGV and polyhedrin gene from SlNPV, they were not closely related to each other. We also found that the two viruses showed similar insecticidal activity against 2nd instar larvae of Spodotera litura in terms of dose-response, but SlGV showed much longer LT50 than that of SlNPV. The two baculoviruses might be cooperatively be applied as biological control agent for the control of S. litura

• Yong Wang(Department of Agricultural Biotechnology, Seoul National University)
• Jae Young Choi(Research Institute for Agriculture and Life Sciences, Seoul National University)
• Jong Yul Roh(Department of Agricultural Biotechnology, Seoul National University)
• Qin Liu(Department of Agricultural Biotechnology, Seoul National University)
• Xueying Tao(Department of Agricultural Biotechnology, Seoul National University)
• Jong Bin Park(Department of Agricultural Biotechnology, Seoul National University)
• Soo Dong Woo(College of Agriculture, Life and Environment Sciences, Chungbuk National University)
• Byung Rae Jin(College of Natural Resources and Life Science, Dong-A University)
• Yeon Ho Je(Department of Agricultural Biotechnology, Seoul National University)