This study aimed to evaluate the antioxidant activity of chestnut honey which were harvested at various areas in South Korea. First at all, we measured the total phenols content through a spectrophotometric determination with a modified Folin-Ciocalteu method and total flavonoids content determined with aluminium chloride. Total phenolic compounds was highest in Sunchang of Chestnut honey(2.21mg/ml)and flavonoids contents was also the highest in Sunchang of Chestnut honey(1.02mg/ml) than other samples. For measured the antioxidant activity of chestnut honey, we performed DPPH(2,2-diphenyl-1-picrylhydrazyl) test and FRAP(ferric reducing-antioxidant assay)test. DPPH scavenging activity highest in Sunchang of Chestnut honey more than 50% DPPH scavenging activitywhile other samples (Gong-ju, Yechen, Chung-ju, Imsil, Ha-dong) showed more than 25% DPPH scavenging activity. The ferric reducing-antioxidant assay (FRAP) is based on the reduction of ferric 2,4,6-tris(2-pyridyl)-1,3,5-triazine [Fe(III)-TPTZ] by spectrophotometric analysis. Sunchang were found to have more than 532μM FRAP activity while other samples (Gong-ju, Yechen, Chung-ju, Imsil, Ha-dong) showed more than 300μM FRAP activity. The results suggested that chestnut honey strong antioxidant activity and it could be utilized as a source of natural antioxidant.