This research evaluate antioxidant and skin-whitening effect of Abeliophyllum distichum Nakai by extraction processes. First, antioxidant effects were follows: EE (70% ethanol extract) showed higher DPPH scavenging activity of 69.66% than WE (hot water exract) 59.13% at 0.3 ㎎/㎖, also UE's (70% ethanol extract by sonication process) higher than EE. Reducing power was that also EE showed higher than WE, and it was the highest value with UE's because of ultrasonic pretreatment. Next, the whitening effect tyrosinase inhibition activity was measured that EE was 23.88%, WE's was 16.69%, and UE was 23.34%. Ultrasonic pretreatment did not influence to tyrosinase inhibition activity. Cell viability showed low cell toxicity in all groups. UE's inhibited melanin synthesis, 55.1%, that is higher than EE and WE, 52.7% and 39.5%, respectively. As a result, we confirmed that antioxidant activities and skin-whitening effect by extraction process. Also, this results confirmed that the Abeliophyllum distichum Nakai extracts worth as cosmetic materials.

서 언
 재료 및 방법
  1. 시료 제조
  2. 세포 및 시약
  3. 추출 수율
  4. 환원력 측정
  5. 세포생존율 평가
  6. DPPH 자유 라디칼 소거능
  7. Tyrosinase 억제 활성
  8. Melanoma cell을 이용한 melanin 생성 저해
  9. 통계처리
 결과 및 고찰
  1. 추출 수율 확인
  2. 환원력 측정
  3. 세포 생존율 확인
  4. DPPH 자유 라디칼 소거능
  5. Tyrosinase 억제 활성 평가
  6. Melanin 생성량 억제 활성 평가
 REFERENCES

• 김남영(강원대학교 생물의소재공학과) | Nam Young Kim
• 이현용(서원대학교 식품공학과) | Hyeon Yong Lee †Corresponding author