Bacillus agaradhaerens 균주의 xylanase 최적 생산조건을 검토하고 효소를 정제하여 그 특성을 확인하였다. 본 균주 의 xylanase 최적 생산조건은 1% CMC, 2% polypeptone, 0.1% K2HPO4, 0.02% MgSO4, 1% Na2CO3, 1% NaCl, pH 9.0이었으며, 종배양액을 1% (w/w)농도로 접종하여 40oC 에서 24시간 동안 160 rpm의 속도로 진탕배양하였을 때 xylanase를 최대로 생산하였다.
The optimum condition for the production of xylanase from Bacillus agaradhaerens DK-2386 isolated from Korean soil has been studied. The optimized medium composition for the production of xylanase from B. agaradhaerens DK-2386 was 1% (w/v) carboxymethyl cellulose, 2% (w/v) polypeptone, 0.1% (w/v) K2HPO4, 0.02% (w/ v) MgSO4, 1% (w/v) Na2CO3, and 1% (w/v) NaCl. The optimum pH of the medium was 9.0. For the production of xylanase, B. agaradhaerens DK-2386 was cultured under the optimized conditions for 24 h at 40oC with 160 rpm of agitation.