Bacillus agaradhaerens 균주의 xylanase 최적 생산조건을 검토하고 효소를 정제하여 그 특성을 확인하였다. 본 균주 의 xylanase 최적 생산조건은 1% CMC, 2% polypeptone, 0.1% K2HPO4, 0.02% MgSO4, 1% Na2CO3, 1% NaCl, pH 9.0이었으며, 종배양액을 1% (w/w)농도로 접종하여 40oC 에서 24시간 동안 160 rpm의 속도로 진탕배양하였을 때 xylanase를 최대로 생산하였다.

The optimum condition for the production of xylanase from Bacillus agaradhaerens DK-2386 isolated from Korean soil has been studied. The optimized medium composition for the production of xylanase from B. agaradhaerens DK-2386 was 1% (w/v) carboxymethyl cellulose, 2% (w/v) polypeptone, 0.1% (w/v) K2HPO4, 0.02% (w/ v) MgSO4, 1% (w/v) Na2CO3, and 1% (w/v) NaCl. The optimum pH of the medium was 9.0. For the production of xylanase, B. agaradhaerens DK-2386 was cultured under the optimized conditions for 24 h at 40oC with 160 rpm of agitation.

서 론
 재료 및 방법
  Xylanase 활성측정
  Xylanase 생산조건
 결과 및 고찰
  Xylanase 생산에 미치는 배양시간, 온도, 진탕배양속도의영향
  Xylanase 생산에 미치는 pH와 배지성분의 영향
  최적배지에서 배양시간에 따른 xylanase 생산 및 균체의 생산
 요 약
 References

• 최지휘(단국대학교 식품공학과) | Ji-Hwi Choi
• 박영서(가천대학교 식품생물공학과) | Young-Seo Park
• 배동훈(단국대학교 식품공학과) | Dong-Hoon Bai Corresponding author