This paper presents the seismic assessment of the effect of vertical ground motion on the three-story RC building with different geometric configurations considering the vertical-to-horizontal peak ground acceleration (V/H) ratio increases. The effects of a suite of earthquake ground motion records on three-story RC buildings are presented and the results are compared with the case of horizontal only excitation. Interstory drift was considered as a global failure criterion, while the curvature ductility and shear capacity of structural members were monitored to assess failure on a local level. The effect of vertical ground motion on axial force and shear capacity is also investigated.