An alkalophilic microorganism, strain DK1122 producing an alkaline protease was identified. DK1122 was isolated from soil collected in central Korea. The strain DK1122 was Gram-positive, 0.7×2-4 μm in size, and its colony was yellowish white, The strain DK1122 was found to be spore-forming, catalase positive, oxidase positive, caseinolytic, and reduce nitrate to nitrite. The protease was produced aerobically on Horikoshi I agar medium (pH 9.0) with 1% (w/v) skim milk at 40°C for 24 h. Through 16S rRNA gene partial sequencing, the strain DK1122 had the 99.7% sequence similarity to 16S rRNA gene sequence of Bacillus pseudofirmus. Based on the biochemical and physiological properties as well as phylogenetic analysis, the isolated strain was named as Bacillus sp. DK1122. It is expected that Bacillus sp. DK1122 may be a promising candidate for a proudcer of an alkaline protease applicable to the food and detergent industries.

서 론
 재료 및 방법
  호알칼리성 균주의 분리 및 선별
  단백질 가수분해 활성 측정
  균주의 형태적 특성
  균주의 생화학적 특성
  균체의 지방산 조성 분석
  16S rRNA 유전자 염기서열 분석
 결과 및 고찰
  호알칼리성 단백질 분해효소 분비 균주의 선별
  균주의 형태적 특성
  균주의 생화학적 특성
  균체 지방산 조성 분석
  16S rRNA 유전자 염기서열
 요 약
 References

• 이형재(단국대학교 식품공학과) | Hyungjae Lee
• 유지승(단국대학교 식품공학과) | Ji-Seung Yoo
• 박영서(가천대학교 식품생물공학과) | Young-Seo Park
• 배동훈(단국대학교 식품공학과) | Dong-Hoon Bai Corresponding author