논문 상세보기
pp.279-292
생약재 5종(상황버섯, 황금, 단삼, 뽕잎 및 작약)의 물 추출물을 동량으로 혼합한 조성물(MHE-1)과 동일한 추출 수율이 되도록 생약재를 혼합한 후 물 추출한 조성물(MHE-2)의 항산화 및 항염증 활성을 비교 분석하였다. 조성물의 총 페놀 및 플라보노이드 함량은 MHE-2에서 더 많았다. DPPH, ABTS 및 superoxide anion 라디칼 소거활성, Fe+2 킬레이팅 활성, 환원력 및 xanthine oxidase 저해활성 등의 항산화 활성은 MHE-2가 더 우수하였다. 항염증 활성은 LPS에 의한 염증 유발군에 비해 조성물 처리 구에서 유의적으로 NO 생성을 억제하였으며, 100μg/mL 농도로 처리 시 PGE2와 cytokine인 TNF-α, IL-2 및 IL-6의 생성도 유의적으로 감소시켰으나 두 조성물간에 차이는 적었다. 이상의 결과를 볼 때 여러 종류의 생약재를 혼합한 조성물의 생리활성은 원재료를 혼합하여 추출하는 것이 추출물의 생리활 성 증대에 효과적인 것으로 판단된다.
We studied the effects of mixing ratio on the antioxidants and anti-inflammatory activities in the 2 kinds of medicinal herbs composites by addition to Phellinus linteus, Scutellaria baicalensis Georgi, Salvia miltiorrhiza Bunge, Morus alba L. and Paeonia lactiflora Pall. The composite MHE-1 was the mixture by the same weight of each hot water extracts of 5 medicinal herbs, and MHE-2 was the hot water extract for the mixture of Phellinus linteus(100g), Scutellaria baicalensis(13.5g), Salvia miltiorrhiza(13.4g), Morus alba(21.2g) and Paeonia lactiflora(37.6g). The contents of total phenolic compounds and flavonoids were significantly higher in MHE-2 than MHE-1. DPPH, ABTS and superoxide radical scavenging activity, Fe+2 chelating activity, reducing power and xanthine oxidase inhibition activity were higher in MHE-2 than MHE-1. In anti-inflammatory activities, two composites were inhibited generation of the NO than LPS-induced inflammatory treatment in RAW264.7 cells. In 100μ g/mL concentration of composites treatment, PGE2 and pro-inflammatory cytokines such as TNF- α, IL-2 and IL-6 production were significantly reduced than LPS-induced inflammatory treatment but, no significant difference between MHE-1 and MHE-2. These result to the antioxidants and anti-inflammatory activities in the 2 kinds of medicinal herbs composites could be suggest that a extraction by mixing the raw materials is more effective than mixture of each extracts.
