To enhance the bioavailability and bioactivities of mixed herbal medicines (RW), they were fermented with lactic-acid bacteria isolated from kimchi into postbiotics (FRW). Then, from the results of the 16s rRNA sequencing analysis, lactic acid bacteria isolated from kimchi were identified to be of two species, namely Lactobacillus sakei and Leuconostoc mesenteroides. The FRW prepared from the RW were extracted using hot water (HW) and 70% EtOH (EtOH) for comparison of their macrophage-stimulating activities. Based on a comparison of the activities of the FRW extracts, nitric oxide (NO) production of HW was significantly higher than that in EtOH. An analysis of the chemical properties of the extracts showed that HW had higher contents of neutral sugar and uronic acid than EtOH as well as contained a large amount of glucose. In addition, crude polysaccharide (CP) was prepared to enhance the macrophage-stimulating activity. The FRW-CP not only secreted immunostimulatory mediators but also increased the expression of immunostimulatory genes (iNOS, TNF-α, MCP-1, and IL-6). The fractionated FRW-CP contained about 90% neutral sugars, and these sugars were mainly composed of glucose, galacturonic acid, and arabinose. Thus, FRW prepared by fermentation of RW with kimchi lactic acid bacteria were found to be immunostimulatory modulators.

생약재 5종(상황버섯, 황금, 단삼, 뽕잎 및 작약)의 물 추출물을 동량으로 혼합한 조성물(MHE-1)과 동일한 추출 수율이 되도록 생약재를 혼합한 후 물 추출한 조성물(MHE-2)의 항산화 및 항염증 활성을 비교 분석하였다. 조성물의 총 페놀 및 플라보노이드 함량은 MHE-2에서 더 많았다. DPPH, ABTS 및 superoxide anion 라디칼 소거활성, Fe+2 킬레이팅 활성, 환원력 및 xanthine oxidase 저해활성 등의 항산화 활성은 MHE-2가 더 우수하였다. 항염증 활성은 LPS에 의한 염증 유발군에 비해 조성물 처리 구에서 유의적으로 NO 생성을 억제하였으며, 100μg/mL 농도로 처리 시 PGE2와 cytokine인 TNF-α, IL-2 및 IL-6의 생성도 유의적으로 감소시켰으나 두 조성물간에 차이는 적었다. 이상의 결과를 볼 때 여러 종류의 생약재를 혼합한 조성물의 생리활성은 원재료를 혼합하여 추출하는 것이 추출물의 생리활 성 증대에 효과적인 것으로 판단된다.

본 연구에서는 국내에 유통되는 생약 원료에 대하여 유기 용매 추출 시에 돌연변이원성이 나타나는 지에 대한 조사를 실시하였다. 추출방법으로는 70% ethanol로 10가지 생약원료 를 모두 추출하여 돌연변이원성을 Ames test를 이용하여 측 정하였고, 또한 70% ethanol 추출물로부터 dichloromethane 및 ethyl acetate를 이용하여 각각 추출한 분획에 대해서도 조사 를 실시한 결과, 70% ethanol에서는 S9 mix가 첨가된 경우, 황기, 천궁 및 산약에서 돌연변이원성이 나타났고, 70% ethanol 추출물로부터 dichloromethane 및 ethayl acetate로 분획한 것 에서는 당귀, 황기, 천궁, 숙지황 및 산약에서 돌연변이원성 이 나타났다. 위의 모든 생약의 경우, S9 mix가 첨가되지 않 은 경우는 돌연변이원성이 나타나지 않았고, S9 mix가 첨가 한 경우에만 돌연변이원성이 나타나는 것으로 보아, 간접돌 연변이원이 본 시험에서 사용한 유기용매에서 용출되는 것 으로 사료된다.

These experiments were performed to investigate the safety of the three medicinal herbs-Curcuma longa Linne, Paeonia japonica Miyabe, Scutellaria baikalensis George-irradiated with gamma rays in respect of genotoxicity. The methanol-soluble and water-soluble fractions of the methanol-water extracts of the 10 kGy gamma-irradiated herbs were examined in two short-term in vitro tests : (1) Salmonella typhimurium reversion assay (Ames test) in strain TA 98, TA 100 and TA 102 (2) Micronucleus test in cultured Chinese hamster ovary (CHO) cells. No mutagenicity was detected in the two assays with or without metabolic activation. From these results, the safety of the herbs irradiated with gamma rays at practical doses could be revealed in further tests of genotoxicity in vivo, chronic and reproductive toxicity.

국내산 재배 생약류 28종 중 열수 추출물에서 항산화력을 나타낸 것으로 조사된 작약, 목단, 황금, 두충, 시호 그리고 산수유의 열수 추출물 6종을 냉동건조한 후 70% ethanol로 용해하여 ethanol 가용성 획분(ESF)과 ethanol 불용성 획분(EIF)으로 분획하였다. 이 분획물들을 60% linoleic acid에 3, 000ppm씩 가한 후 35℃의 항온기에서 저장하면서 BHA 첨가구와의 산화안정성을 비교하였다. 이 결과 작약과 황금의 ESF는 유도기간이 각각 12일과 9일인데 반해 BHA 첨가구는 9일로 나타나 산화 안정성이 더 높거나 같은 것으로 나타났다. 한편 6종의 생약류의 ESF는 EIF 보다 linoleic acid에 대한 항산화 효과가 모두 높은 것으로 나타났다.

The antimutagenic effects of 46 kinds of medicinal plants that have been used as traditional folk antitumor agents in Korea were studied by using Ames mutagenicity teat. Most of the methanolic extracts from the plants which were used in this experiment showed strong antimutagenic activity toward atlatoxin B₁(AFB₁) in Salmonella typhimurium TA100 and TA98. However, N-methyl-N'-vitro-N-nitrosoguanidine (MNNG) induced mutagenicity was not blocked by adding the methanolic extracts of the plants except persimmon leaves (Diospyros kaki Thunberg) and Elaeagnus umbellata.

본 연구는 당뇨처방을 근거로 구성된 생약재 복합물의 항당뇨 효능을 조사하기 위하여 복합물의 인슐린성 물질을 탐색하고 -amylase 및 -glucosidase 저해활성을 평가하였다. 3T3-L1 세포에 복합 생약재 추출물을 의 농도로 처리하였을 때 지방세포로의 분화능은 151.7%로 인슐린의 작용을 향상시키는 것으로 확인되었다. 혈당저하제로서 생약재 복합 추출물의 -amylase에 대한 저해활성은 생약재 복합 추출물 10.0, 1.0 및 0.1

본 연구는 다양한 생약재를 사용하여 생약재 복합물을 구성하고, 복합물의 이화학적 특성과 항산화 활성에 대하여 조사하였다. 생약재 복합물의 추출 수율은 물 추출물이 35.00%, 에탄올 추출물 25.33%이었으며, 분획물 중에서는 ethyl acetate층의 수율이 물 및 80% 에탄올 추출물에서 각각 0.64% 및 3.76%였다. 생약재 복합물의 물 및 80% 에탄올 추출물의 총플라보노이드 함량은 각각 2.34% 및 2.42%이었고 총페놀성 화합

당뇨병에 효과가 있는 식품소재의 탐색과 그 소재를 이용한 기능성 빵제품 개발에 관한 연구의 일환으로 당뇨병에 효과가 있다고 알려진 소장흡수 저해효소인 -glucosidase를 target로 시중에 유통되는 다양한 생약재를 대상으로 탐색을 하였다. -Glucosidase 저해활성을 조사하고자 생약재 200여종을 대상으로 물층과 메탄올 층으로 구분하여 탐색한 결과 물층에서는 황기(70.9%)가, MeOH층에서는 구인(72.9%)이 70%이상의 강력한

Various herbal medicinal extracts were examined for the development of cosmetic products with anti-oxidative and anti-wrinkle activity. First, total polyphenol contents and DPPH radical scavenging activities of herbal medicinal extracts were measured. Most herbal samples, except for DW extracts of Portulaca oleracea, Caesalpinia sappan, Taraxacum platycarpum, Carthamus tinctorius, and 70% EtOH extracts of Taraxacum platycarpum and Carthamus tinctorius, showed DPPH radical scavenging activity over 80% at a concentration of 400μg/ml. SOD-like antioxidant activity of DW extracts of Syzygium aromaticum, DW extracts of Eriobtrya japonica and 70% EtOH extracts of Sophora japonica was measured as 40%, 35% and 80%, respectively at a dry matter concentration of 50μg/ml. In elastase inhibition assay, DW extracts of Lycium chinense (50μg/ml) and 70% EtOH extracts of Areca catechu (50μg/ml) showed 50% and 40% of inhibition, respectively. At a concentration of 1.250μg/ml, DW extracts of Lycium chinense and 70% EtOH extracts of Areca catechu showed 10% and 30% of collagenase inhibition, respectively. Skin and lotion samples were prepared using the two herbal extracts of high anti-wrinkle activity: Lycium chinense extract and Areca catechu extract. The storage stability of skin and lotion containing each of the selected herbal extracts was evaluated. pH and viscosity were used as stability indicators for the stability test under different storage temperatures and freeze-thaw cycle conditions. The skin and lotion containing each of DW extract of Lycium chinense and 70% EtOH extract of Areca catechu was showed high pH and viscosity stability. The skin and lotion containing DW extracts of Lycium chinense showed relatively higher stability than the skin and lotion containing 70% EtOH extract of Areca catechu, at cycle chamber and freeze-thaw conditions. In summary, these results indicated that cosmetics containing DW extract of Lycium chinense were relatively stable, and this herbal extract could be used as a stable functional cosmetic material.

Eleuthero(Eleutherococcus senticosus Maxim.) cortex is well known as a herb medicine for tonic. This study was performed to improve the quality of dried E. senticosus cortex. Investigation of quality factor and contents of efficient compounds under different steaming times and drying methods were performed to determine the proper processing and drying conditions of Eleuthero cortex harvested on March in annual stems. The proper steaming time for peeling bark to make high quality Eleuthero cortex took less than 20 mins. Eleutheroside B and E contents among drying methods were significantly different at 5% level DMRT. The 50℃ heat drying was the most advisable condition for drying, when drying and keeping contents of effective compounds.

생약재의 용매별 추출액에 대한 생리활성 측정 결과 총폴리페놀 함량은 모든 생약재가 50 ethanol 추출액에서 가장 우수하였으며, 생약재 별로는 감초의 50 ethanol 추출액이 2.23로 가장 높은 함량을 나타내었다. 전자공여 작용은 감초 water 추출액이 81.81로 가장 우수하였으며, 감초, 건강는 water 추출액이 두충, 건마늘, 인삼은 50 ethanol 추출액에서 다른 용매에 비해 높은 전자공여 효과를 나타내었다. Superox

These experiments were performed to investigate the safety of two herbs-Houttuynia cordata Thunberg and Lycium chinese Miller-irradiated with gamma-rays in respect of genotoxicity. Water extracts from the 10 kGy gamma-irradiated herbs were examined in two short -term in vitro tests ; (1) Salmonella typhimurium reversion assay (Ames test) in strain TA 98 and Ta100 and (2) Micronuclues test on clutured Chinese hamster ovary(CHO) cells. No mutagenicity was detected in the two assays with or without metabolic activation . From these results , the safety of the herbs irradiated with gamma-rays at practical doses could be revealed in further tests of genotoxicity in vivo, chronic and reproductive toxicity.