Electrolysis techniques(electrokinetic) generally have been used mainly to remove the inorganic salts like Cu, Pb etc. in the soil. In the organic living, the effects of electrolysis has a direct impact on survival by dissociation of the ions inside the cell and accumulation of energy within cells. In this study, It was evaluated for susceptibility to nematodes(Meloidgyne javanica, Pratylenchus coffeae) in accordance with the voltage, current and electrolyte concentration. Using the electrolytic bath and electrolyte material(TAE buffer, Nacl), the susceptibility of nematodes was evaluated in the range of 30 ~ 60V, 0.02 ~ 0.77A. Unusually, the high voltage and amperage did not increase the death rate of nematodes and the most resistant stage was not clear because sensitivity of each stage could not be distinguished. In 30V 0.52A, 0.70A, 0.72A, M. javanica eggs processed for 10 minutes did not hatch at all. In 30V 0.58A, M. javanica juveniles processed for 5 minutes were showed 100% mortality and in 30V 0.77A, P. coffeae juveniles processed for 5 minutes were showed highest sensitivity compared to other treatment range.