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pp.161-173
본 연구에서는 α-glucosidase 저해활성이 우수한 천연 자원을 개발하기 위하여 개똥쑥으로부터 천연 물질을 분리·동정하고 분리된 물질에 대하여 α-glucosidase 저해활성을 측정하였다. 개똥쑥 잎과 줄 기의 chloroform층과 ethyl acetate층으로부터 각각 6개와 5개의 화합물을 분리하였다. 분리한 화합물 중 chloroform층으로부터 분리된 ArteCA(M.W. 316g/mol, C16H12O7)는 isorhamnetin, ArteCB(M.W. 360g/mol, C18H16O8)는 chrysosplenol D, ArteCC(M.W. 316 g/mol, C16H12O7)는 rhamnetin, ArteCD (M.W. 374g/mol, C18H16O8)는 chrysosplenetin, ArteCE(M.W. 284g/mol, C21H20O12)는 acacetin 그리 고 ArteCF(M.W. 270g/mol, C16H14O4)는 imperatorin으로 확인되었다. 한편 EtOAc층으로부터 분리된 ArteEAA(M.W. 178g/mol, C9H6O4)는 6,7-dihydroxy coumarin, ArteEAB (M.W. 192g/mol, C10H8O4) 는 scopoletin, ArteEAC (M.W. 448g/mol, C21H20O11)는 kaempferol-3-O-b-D-glucoside, ArteEAD (M.W. 464 g/mol, C21H20O12)는 quercetin-3-O-b-D-glucoside 및 ArteEAE(M.W. 318g/mol, C15H10O8)는 myricetin으로 확인되었다. 이 중에서 ArteEAE(myricetin)는 α-glucosidase에 대하여 97.3%의 높은 저해활성을 가지고 있었으므로, 혈당조절용 건강식품 또는 치료제 개발을 위한 물질로 활용될 수 있을 것으로 판단되었다.
The purpose of this study is to identify and develop a natural resource with good α-glucosidase inhibitory activity. To this end, natural substances were separated and developed from Artemisia annua L., and its α-glucosidase inhibitory activity was measured. From Artemisia annua L. leaves and stems of the chloroform layer and the ethyl acetate layer, 6 and 5 compounds were separated, respectively. From the chloroform layer, the separated compounds were identified as follows: ArteCA(M.W. 316g/mol, C16H12O7) as isorhamnetin, ArteCB(M.W. 360 g/mol, C18H16O8) as chrysosplenol D, ArteCC(M.W. 316g/mol, C16H12O7) as rhamnetin, ArteCD(M.W. 374 g/mol, C18H16O8) as chrysosplenetin, ArteCE(M.W. 284g/mol, C21H20O12) as acacetin, and ArteCF(M.W. 270g/mol, C16H14O4) as imperatorin. From the ethyl acetate layer, the separated compounds were identified as follows: ArteEAA(M.W. 178g/mol, C9H6O4) as 6,7-dihydroxycoumarin, ArteEAB(M.W. 192g/mol, C10H8O4) as scopoletin, ArteEAC(M.W. 448g/mol, C21H20O11) as kaempferol-3-b-D-glucoside, ArteEAD(M.W. 464 g/mol, C21H20O12) as quercetin-3-b-D-glucoside, ArteEAE(M.W. 318g/mol, C15H10O8) as myricetin. Among these compounds, the ArteEAE(myricetin) shows a high level of α-glucosidase inhibitory activity (98.1%). Thus, this could be applied in research and development for blood sugar control supplements or medications. Furthermore, additional research is needed to demonstrate the effects and functions of 11 compounds separated from the leaves and stems of Artemisia annua L.
재료 및 방법
결과 및 고찰
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