To elucidate occurrence patterns of C4 plants in agroecosystems, we surveyed flora inside fields and embankments in 222 sites in farming lands in summer and fall, 2013. We also observed Kranz anatomy and analyzed carbon stable isotope to classify photosynthesis types. Our study observed total 54 species of C4 plants in 33 genera and 9 families of vascular plants besides cultivated crops occurring in farming fields. The most common species was Portulaca oleracea L. followed by Digitaria ciliaris and Echinochloa crusgalli (L.) P. Beauv. var. crusgalli among the C4 plants. The ratios of C3 and C4 species number to total number of species tended to maintain constant regardless of survey times and habitat types. The proportion of C4 species to the total number of species were more influenced by the number of C3 species than those of other types in the surveyed sites. Sizes of cultivation areas did not affect the proportions of C4 plants. Increasing number of simultaneous weeding methods, especially with cutting method, appeared to decrease the number of C3 plants. On the other hand, as the number of cultivated crops increased, both C3 and C4 plants increased but the proportions of C4 species remained. We interpret our results that proportion of C4 species in agroecosystems, appear to be dependent on the number of C3 species, which in turn, appear to be affected by cultivation methods.