Purpose: The study aim was to understand the experiences of intensive care unit (ICU) nurses in labor union activities. Methods: A descriptive qualitative study and content analysis were conducted. The participants included nine ICU nurses who shared their experiences with labor union activities. The data were collected through in-depth interviews. Results: The experiences of ICU nurses with labor union activities encompassed five themes: “The Initial Step in Union Activities”, “Benefits Derived from Union Participation”, “Concerns Regarding Union Activities”, “The Non-Omnipotence of Unions”, and “Achieving Mutual Benefits through Union Activities”. Conclusion: The study findings revealed that ICU nurses strive to improve their work and the nursing environment through labor unions, despite facing negative perceptions of union involvement.

This study investigated the effect of Lactiplantibacillus plantarum JSA22-fermented rice drinks on dextran sodium sulfate (DSS)-induced colitis in mice. Twenty-four mice were randomly assigned; No colitis (Con), colitis with tap water (DSS-only), colitis with unfermented rice (DSS-UFR), and colitis with fermented rice (DSS-FR). After inducing colitis with 2% DSS for 5 days, they were given Tap water, UFR drink, or FR drink for an additional 6 days. The DSS-FR group had significantly lower Disease Activity Index (DAI) scores compared to the DSS-only group, but no significant difference with the DSS-UFR group. Colon length was reduced in the DSS-only group. The DSS-only group had significantly higher IL-6 mRNA levels compared to the Con group, while the DSS-FR groups showed significantly lower IL-6 mRNA levels compared to the DSS-only group. These results suggest that rice drinks fermented with Lactiplantibacillus Plantarum JSA22 ameliorate the severity of DSS-colitis, by potentially reducing proinflammatory cytokines.

Dissolved organic matter (DOM) is a key component in the biogeochemical cycling in freshwater ecosystem. However, it has been rarely explored, particularly complex river watershed dominated by natural and anthropogenic sources, such as various effluent facility and livestock. The current research developed a new analytical method for TOC/TN (Total Organic Carbon/Total Nitrogen) stable isotope ratio, and distinguish DOM source using stable isotope value (δ13C-DOC) and spectroscopic indices (fluorescence index [FI] and biological index [BIX]). The TOC/TN-IR/MS analytical system was optimized and precision and accuracy were secured using two international standards (IAEA-600 Caffein, IAEA-CH-6 Sucrose). As a result of controlling the instrumental conditions to enable TOC stable isotope analysis even in low-concentration environmental samples (<1 mgC L-1), the minimum detection limit was improved. The 12 potential DOM source were collected from watershed, which includes top-soils, groundwater, plant group (fallen leaves, riparian plants, suspended algae) and effluent group (pig and cow livestock, agricultural land, urban, industry facility, swine facility and wastewater treatment facilities). As a result of comparing characteristics between 12 sources using spectroscopic indices and δ13C-DOC values, it were divided into four groups according to their characteristics as a respective DOM sources. The current study established the TOC/TN stable isotope analyses system for the first time in Korea, and found that spectroscopic indices and δ13C-DOC are very useful tool to trace the origin of organic matter in the aquatic environments through library database.

Purpose: This study utilizes a phenomenological research method to understand the nature of role adaptation experience of PA nurses. Methods: This was a qualitative study that applied Colaizzi’s phenomenological research method to understand the nature of role adaptation experience of PA nurses. The study participants were thirteen PA nurses (three men and ten women) with over five years of experience. For data collection, individual in-depth interviews were conducted, each about 50 to 90 minutes long. Individual interviews were conducted once or twice. Colaizzi’s method was used to analyze data. Results: The result of this study had 11 clusters of themes and 4 major themes were derived from the analysis: “parallel lines of conflict,”“anxiety about unclear future,”“disorganization of identity,”and “my own way to learn about myself.”These four major themes are structured chronologically to the extent possible but are not mechanically segmented based on time. Conclusion: This study recognizes the seriousness of PA nurses’problems in the absence of research on PA nurses’role adaptation experience and recommends that appropriate research is necessary. Standardized work guidelines and positive awareness of the role of PA nurses are necessary and are supported by PA nurses to establish their position as professionals.

우리나라는 삼면이 바다로 이루어져 있고, 이에 따라 많은 해양 시설로 인한 위험유해물질이 배출되고 있으나, 배출관리 및 규제 시스템이 미비한 상황이다. 따라서, 위험유해물질(HNS) 관리를 위하여 효율적으로 데이터를 수집할 수 있는 시스템이 필요하 다. 본 연구에서는 HNS 데이터를 효율적으로 관리 및 저장하기 위한 데이터 표준화 시스템을 설계하고 이의 표준화 방안을 제시하고 자 한다.

고준위폐기물을 심지층에 처분하기 위한 공학적방벽의 구성 요소로는 처분용기, 완충재, 뒷채움재 등이 있다. 이 중 완충재는 처분용기와 근계암반 사이의 빈 공간에 설치되는 물질로써, 주변 지하수로부터 처분용기를 보호하며 방사성 핵종의 유출을 저지하는 등의 역할을 한다. 또한 처분용기에서 발생하는 고온의 열량은 완충재로 직접 전파되기에 완충재의 열전도도는 처분시스템의 안전성 평가에 있어 매우 중요하다고 할 수 있다. 따라서 본 연구에서는 국내 경주산 압축 벤토나이트 완충재의 열전도도 특성을 규명하였으며 실제 처분용기에서 발생되는 고온의 특성을 반영하여 상온에서 80~90℃까지의 범위에서 압축 벤토나이트의 열전도도를 측정하였다. 온도증가에 따라 압축 벤토나이트의 열전도도는 5~20% 가량 증가하였으며 초기 포화도가 클수록 열전도도 증가는 더 크게 나타났다.

인산염 농도 변화에 따른 Hizikia fusiforme의 무기비소 축적량 및 생장률을 분석하였다. 무기비소에 14일간 노출 하였을 때, 2 mg L-1의 높은 인산염 농도에서 무기비소 축적량이 증가하지 않았다. 하지만 인산염 농도가 0.02 mgL-1로 낮은 경우에 무기비소 축적량이 3배 이상 증가하였다. 또한 H. fusiforme는 인산염 농도가 낮은 경우 생장률이 14.5%, 무기비소 (10 μg L-1)에 노출되었을 경우 생장률이 대조구 대비 30% 감소하였다. H. fusiforme는 인산염과 무기비소를 구분하지 못하여 인산염의 농도가 낮은 경우 무기비소 축적량이 증가하게 되고, 축적된 무기비소는 광합성 저해 및 세포분열을 방해하여 생장률을 억제한다. 특히 우리나라의 대표적인 양식생물인 H. fusiforme는 다른 해조류에 비해 상대적으로 무기비소 축적량이 높다고 알려져 있기 때문에, H. fusiforme의 식품안전성을 확보하기 위해 다양한 연구가 필요하다.

Stable isotope tracers were first applied to evaluate the Microcystis cell assimilation efficiency of bivalves, since the past identification method has been limited to tracking the changes of each chl-a, clearity, and nutrient. The filter-feeders (Sinanodonta woodiana and Unio douglasiae) were assessed under the condition of cyanobacteria (Microcystis aeruginosa) blooms through an in mesocosm experiment using 13C and 15N dual isotope tracers. Chl-a concentration in the treatment mesocosm was dramatically decreased after the beginning of the second day, ranging from 116 to 66 μg L−1. In addition, the incorporated 13C and 15N atom % in the S. woodiana bivalve showed higher values than U. douglasiae bivalves. The results demonstrate that U. douglasiae has less capacity to assimilate toxic cyanobacteria derived from diet. Our results therefore also indicate that S. woodiana can eliminate the toxin more rapidly than U. douglasiae, having a larger detoxification capacity.

정부는 기후변화로 인하여 변화하고 있는 해양생태계를 보전하기 위해 노력하고 있다. 이와 관련하여 정부는 해양생태계를 보전하고 관리하기 위하여 보호대상해양생물 77종을 지정하여 특별히 관리하고 있다. 산호의 경우 해양생태계를 예측할 수 있는 중요한 생물군 중 하나이며, 해양생물에게는 서식지를 제공하는 매우 중요한 역할을 하고 있다. 인간의 다양한 활동으로 인해 산호는 위협받고 있으며, 이에 대한 보전과 관리가 시급하다. 따라서 산호의 가치를 평가하여 이해하는 것은 정책 입안자 및 일반 대중 모두에게 산호의 지속적인 관리의 혜택을 입증 할 수 있는 효과적인 방법이 될 수 있다. 따라서 본 연구에서는 조건부 가치측정법을 적용하여, 국내 대표 산호군락지인 문섬 등 주변해역 생태계보호구역 내 산호군락지의 보전가치를 평가해보고자 한다. 이를 위해 전국 1,000가구를 대상으로 일대일 개별면접을 실시하여, 문섬 등 주변해역 생태계보호구역 내 산호군락지의 보전을 위한 대중의 지불의사를 평가하였다. 질문법으로 1.5경계 양분선택형 모형을 활용하였으며, 0의 WTP를 처리하기 위해 스파이크 모델을 적용하였다. 그 결과 문섬 등 주변해역 생태계보호구역 내 산호군락지의 보전을 위한 가구당 연간 평균 WTP는 3,016원이었으며, 유의수준 1%에서 통계적으로 유의했다. 이 값을 모집단 전체로 확장하면, 문섬 등 주변해역 생태계보호구역 내 산호군락지의 보전가치가 연간 589억원으로 계산된다. 본 연구는 산호군락지 보전을 위해 국민들이 상당한 금액을 기꺼이 지불 할 의향이 있다고 판단하였다.

Nitrogen (N) loading from domestic, agricultural and industrial sources can lead to excessive growth of macrophytes or phytoplankton in aquatic environment. Many studies have used stable isotope ratios to identify anthropogenic nitrogen in aquatic systems as a useful method for studying nitrogen cycle. In this study to evaluate the precision and accuracy of denitrification bacteria method (Pseudomonas chlororaphis ssp. Aureofaciens (ATCC® 13985)), three reference (IAEA-NO-3 (Potassium nitrate KNO3), USGS34 (Potassium nitrate KNO3), USGS35 (Sodium nitrate KNO3)) were analyzed 5 times repeatedly. Measured the δ15N-NO3 and δ18O-NO3 values of IAEA-NO-3, USGS 34 and USGS35 were δ15N: 4.7±0.1‰ δ18O: 25.6±0.5‰, δ15N: -1.8±0.1‰ δ18O: -27.8±0.4‰, and δ15N: 2.7±0.2‰ δ18O: 57.5±0.7‰, respectively, which are within recommended values of analytical uncertainties. Also, we investigated isotope values of potential nitrogen source (soil, synthetic fertilizer and organic-animal manures) and temporal patterns of δ15N-NO3 and δ18O-NO3 values in river samples during from May to December. δ15N-NO3 and δ18O-NO3 values are enriched in December suggesting that organic-animal manures should be one of the main N sources in those areas. The current study clarifies the reliability of denitrification bacteria method and the usefulness of stable isotopic techniques to trace the anthropogenic nitrogen source in freshwater ecosystem.

The nitrogen isotope value in both ammonium and nitrate ion were determined at 9 stations during both June and August 2016, in order to understand the origin of DIN at the Han river. δ15N-NO3 and δ15N-NH4 values in 8 stations (CP, SB, MHC, P4, SJ, SBC, P2, SC) were no significant variation. However δ15N-NO3 and δ15N-NH4 values in KK (Kyeongan stream) showed significant different in comparison with 8 stations, with an apparent increase of nitrogen isotope values. These results indicate that antropogenic nitrogen source influence on KK station. Also the δ13C and δ15N isotope ratio of phytoplankton (Diatom and Cyanobacteria) in KK (Kyeongan stream) showed heavier values, compared to other study stations. These results indicate that nitrogen isotope value in phytoplankton effects by different nitrogen source in study sites. These results suggest that the analysis of stable isotope ratios is a simple but useful tool for the identification of dissolved inorganic nitrogen origin in aquatic environments.

Fludarabine, a chain terminating anti-cancer drug, is a purine analogue that causes DNA strand breaks in normal cells. In this study, we determined if A. melanocarpa and Korean red ginseng extract mixture reduce cytotoxicity of fludarabine. Treatment of HaCaT cells with 10 μM of fludarabine for 24 hours decreased cell viability and increased DNA strand breaks. Treatment of A. melanocarpa and Korean red ginseng extract mixture for 24 hours increased cell viability as compared with single extract treatment. The protective effect of these extracts on cell activity increased in a concentration-dependent manner. DNA strand breaks induced by fludarabine decreased as concentration of extract mixture increased. p-H2AX level, a marker of DNA strand breakage, decreased depending on the concentration of extract mixture. The effect of mixed extract of A. melanocarpa and Korean red ginseng on DNA damage is due to the anti-oxidative effect of A. melanocarpa and signal transmission through glucocorticoid receptor upon binding of saponin of Korean red ginseng.

본 논문에서는 푸른바다거북의 구조・치료를 통한 보전의 공익적 가치를 경제학적 방법론에 근거하여 평가하고자 조건부가치측정법을 적용하였다. 자료의 수집은 여론조사전문기관이 무작위 추출한 전국 1,000 가구를 대상으로 일대일 개별면접조사를 통해 이루어졌다. 지불의사 유도방법으로 1.5경계 모형을 적용하되 지불수단으로는 가구당 소득세를 활용하였다. 지불의사액 분석모형으로는 효용격차모형에 근거한 스파이크 모형을 적용하였다. 분석결과, 응답가구는 중앙정부가 푸른바다거북 구조, 치료, 방류를 시행하기 위해 2016년말 기준 향후 10년 동안 매년 평균적으로 2,570원을 부담하고자 하였으며 이 값은 유의수준 1%에서 통계적으로 유의하였다. 이 값을 전국으로 확장하면 푸른바다거북 보전의 공익적 가치가 연간 487억원임을 알 수 있다. 따라서 중앙정부 차원에서 어업활동 중의 부수어획, 어선과 충돌, 폐그물에 걸려 죽거나, 부상당한 푸른바다거북에 대해 구조, 치료, 방류를 추진하는 것의 공익적 가치는 작지않다.

Stable Isotope Analysis (SIA) of carbon and nitrogen is useful tool for the understanding functional roles of target organisms in biological interactions in the food web. Recently, mixing model based on SIA is frequently used to determine which of the potential food sources predominantly assimilated by consumers, however, application of model is often limited and difficult for non-expert users of software. In the present study, we suggest easy manual of R software and package SIAR with example data regarding selective feeding of crustaceans dominated freshwater zooplankton community. We collected SIA data from the experimental mesocosms set up at the littoral area of eutrophic Chodae Reservoir, and analyzed the dominant crustacean species main food sources among small sized particulate organic matters (POM, <50 μm), large sized POM (>50 μm), and attached POM using mixing model. From the results obtained by SIAR model, Daphnia galeata and Ostracoda mainly consumed small sized POM while Simocephalus vetulus consumed both small and large sized POM simultaneously. Copepods collected from the reservoir showed no preferences on various food items, but in the mesocosm tanks, main food sources for the copepods was attached POM rather than planktonic preys including rotifers. The results have suggested that their roles as grazers in food web of eutrophicated reservoirs are different, and S. vetulus is more efficient grazer on wide range of food items such as large colony of phytoplankton and cyanobacteria during water bloom period.