Dissolved organic matter (DOM) is a key component in the biogeochemical cycling in freshwater ecosystem. However, it has been rarely explored, particularly complex river watershed dominated by natural and anthropogenic sources, such as various effluent facility and livestock. The current research developed a new analytical method for TOC/TN (Total Organic Carbon/Total Nitrogen) stable isotope ratio, and distinguish DOM source using stable isotope value (δ13C-DOC) and spectroscopic indices (fluorescence index [FI] and biological index [BIX]). The TOC/TN-IR/MS analytical system was optimized and precision and accuracy were secured using two international standards (IAEA-600 Caffein, IAEA-CH-6 Sucrose). As a result of controlling the instrumental conditions to enable TOC stable isotope analysis even in low-concentration environmental samples (<1 mgC L-1), the minimum detection limit was improved. The 12 potential DOM source were collected from watershed, which includes top-soils, groundwater, plant group (fallen leaves, riparian plants, suspended algae) and effluent group (pig and cow livestock, agricultural land, urban, industry facility, swine facility and wastewater treatment facilities). As a result of comparing characteristics between 12 sources using spectroscopic indices and δ13C-DOC values, it were divided into four groups according to their characteristics as a respective DOM sources. The current study established the TOC/TN stable isotope analyses system for the first time in Korea, and found that spectroscopic indices and δ13C-DOC are very useful tool to trace the origin of organic matter in the aquatic environments through library database.

A new clamped mechanical splice system was proposed to develop structural performance and constructability for precast concrete connections. The proposed mechanical splice resists external loading immediately after the engagement. The mechanical splices applicable for both large-scale rebars for plants and small-scale rebars for buildings were developed with the same design concept. Quasi-static lateral cyclic loading tests were conducted with reinforced and precast concrete members to verify the seismic performance. Also, shaking table tests with three types of seismic wave excitation, 1) random wave with white noise, 2) the 2016 Gyeongju earthquake, and 3) the 1999 Chi-Chi earthquake, were conducted to confirm the dynamic performance. All tests were performed with real-scale concrete specimens. Sensors measured the lateral load, acceleration, displacement, crack pattern, and secant system stiffness, and energy dissipation was determined by lateral load-displacement relation. As a result, the precast specimen provided the emulative performance with RC. In the shaking table tests, PC frames’ maximum acceleration and displacement response were amplified 1.57 - 2.85 and 2.20 - 2.92 times compared to the ground motions. The precast specimens utilizing clamped mechanical splice showed ductile behavior with energy dissipation capacity against strong motion earthquakes.