본 연구는 식물공장에서 미나리(Oenanthe javanica DC.) 를 초장별(20cm, 30cm, 40cm, 50cm)로 재배할 경우 기능성 물질과 영양학적 가치가 유지되는지를 평가하기 위해 수행되 었다. 미나리는 항산화 효과, 항염증, 간 보호 등 다양한 생리 활성을 갖춘 기능성 채소로, 기존 재배 방식의 한계를 극복하 고 연중 안정적인 생산이 가능한 식물공장 재배 가능성을 확 인하는 것이 본 연구의 목적이다. 연구 결과, 초장 30cm에서 칼륨(K)과 칼슘(Ca) 함량이 가장 높았으며, 비타민 C 함량 또 한 1,165.38mg·kg-1으로 가장 높았다. 총 폴리페놀 함량은 초 장 20－40cm에서 유사한 수준을 유지하였으며, 초장 50cm 에서 감소하는 경향을 보였다. 기능성 물질인 Quercetin, Apigenin, Isorhamnetin 함량은 초장에 따른 변동 폭이 크지 않아, 단기 재배된 초장이 짧은 미나리도 충분한 기능성 물질 을 함유하고 있음을 확인하였다. 식이섬유 함량 또한 모든 초 장에서 일정 수준(300g·kg-1 이상)을 유지하여 건강 기능성 채소로 활용 가능성이 높았다. 본 연구 결과를 종합하면, 식물 공장에서 초장 30cm로 재배된 미나리는 높은 영양 가치와 기 능성을 유지할 수 있으며, 기존 노지재배 방식과 비교해 연중 균일한 생산이 가능하여 산업적 활용 가치가 클 것으로 판단 된다.

본 연구는 식물공장형 육묘시스템에서 광도 조절에 따른 수 박 접목묘의 생육 및 품질을 평가하기 위해 수행되었다. 수박 접수는 고온기 품종 ‘부라보꿀(BK)’, 저온기 품종 ‘산타꿀 (SK)’을 사용하였고, 대목으로 고온기 품종 ‘불로장생(BS)’, 저온기 품종 ‘동장군(DG)’을 사용하였다. 광도는 125 ± 25, 225 ± 25 및 325 ± 25μmol·m-2·s-1 3처리로 조정하여 실험을 진행하였다. 초장은 고온기 접목묘가 125 ± 25μmol·m-2·s-1, 저온기 접목묘가 225 ± 25μmol·m-2·s-1에서 가장 높았고, 줄 기 직경과 마디수는 고온기 접목묘, 저온기 접목묘 모두 225 ± 25μmol·m-2·s-1에서 가장 높았다. 근장과 엽면적은 고온기 접 목묘에서 325 ± 25μmol·m-2·s-1, 저온기 접목묘는 225 ± 25 μmol·m-2·s-1에서 가장 높았다. 또한 지상부 및 지하부 생체중 과 건물중은 225 ± 25μmol·m-2·s-1 처리에서 가장 높게 나타 났다. 충실도는 두 품종 모두 광도가 증가함에 따라 높아졌으 며, 특히 고온기 접목묘에서 그 효과가 두드러졌다. 엽면적비 (LAR)는 고온기 접목묘에서 광도 증가와 함께 유의하게 증가 하였으나, 저온기 접목묘에서 유의한 차이가 없었다. 종합적 으로 생육 특성은 225 ± 25μmol·m-2·s-1에서 높았으나, 묘소 질을 고려했을 때 325 ± 25μmol·m-2·s-1에서 더욱 강건한 묘 가 형성된 것으로 판단된다.

본 연구는 지난 10여년간 고려대학교 및 한국과학기술연구원(KIST)에서 AMS를 이용하여 측정된 우주선유발 동위원소 10 Be의 준비 및 측정 과정을 다룬 보고서이다. 총 2 4세트의 표준물질과 107개의 Blank시료가 분석되었으며, 2017년 이후 표준물질의 측정 재현성이 크게 향상되어 국제적인 주요 실험실의 결과와 비교할 만한 수준의 성과를 보였다. 그러나 Blank 값은 사용된 9 Be 캐리어의 종류 및 석영 추출 과정과 AMS 측정 세팅 등 실험 조건에 따라 영향 을 받는 것으로 나타났으며, 이는 잠재적인 오염원을 체계적으로 추적하고 관리할 필요성을 시사한다. 본 연구는 10 Be Background 값을 줄이는 것이 우주선유발 동위원소 연구의 범위를 넓히는 데 중요한 역할을 함을 강조한다. 낮은 Background 값은 상대적으로 최근에 형성된 지형이나 오랜 시간 매몰되어 방사성 핵종이 상당량 감소한, 저준위 10 Be 지형의 연대측정을 가능하게 한다. 향후 연구에서는 실험 과정을 개선하고 가중평균 계산법 등의 새로운 통계적 방법을 도입하여 측정 오차를 최소화하는 데 중점을 둘 예정이다. 본 연구는 다양한 시간적 및 공간적 규모에서 지구표면과학 연구를 발전시키기 위한 우주선유발 동위원소 분석의 기반을 제공한다.

This study aims to propose new grading standards that can be applied to AI-based automatic sorting machines, reflecting current distribution and consumption trends. The current domestic grading standards for onions in South Korea are based on the “Agricultural and Fishery Products Quality Control Act”. They classify onions based on criteria such as uniformity, shape, color, and the presence of foreign matter. Onion grading standards are divided into four categories based on bulb diameter and weight. However, in the actual domestic market, onions are distributed according to a five-grade classification based on bulb diameter. Therefore, this study classified onions into eight grades, reflecting current distribution and consumption trends in the domestic market. These grades are applicable to AI-based automatic sorting machines. Marketable onions were classified into A1 (extra large) to A5 (extra small) based on the diameter of a single bulb. Onions used for non-marketable purposes (processing) were classified as grade B. Additionally, grade C and grade D were designated for processing and disposal, respectively. By establishing quality grading classifications that align with current distribution and consumption market trends as well as the operational characteristics of AI-based automatic sorting machines, we can expect improvements in work efficiency and reductions in distribution costs. Following this study, it will be necessary to establish comprehensive quality grading standards that include both external criteria (such as bulb weight and size) and internal criteria (such as detection of internal decay and disease occurrence).

The purpose of this study is to estimate the production function of the oliver flounder (Paralichthys olivaceus) aquaculture industry, analyze its efficiency, and provide implications for sustainable aquaculture. For this purpose, the SFA model was utilized. The results of this study are as follows. First, the coefficient values of labor wages and farm area are higher than other inputs. This suggests that the Korean oliver flounder aquaculture industry is labor-intensive. Second, disease management is highly influenced by management efficiency. The more frequently farms are disinfected and culled, the higher the management efficiency with culling having a greater impact on management efficiency. Finally, the cost inputs of high and low-efficiency fish farms were analyzed, and it is recommended that wages, electricity, drugs, and oxygen should be kept at a reasonable level rather than excessive. However, the higher the inputs for mortality removal and disease management, the higher the management efficiency. In other words, improving the farm environment is the most important to achieve sustainability and management efficiency of Korean oliver flounder farming.

최근 지구온난화를 동반한 기후변화가 가속화되고 있으며, RCP 8.5 시나리오에 따르면 21세기 말까지 연평균 기온은 4.8℃ 상승할 것으로 예상된다. 고온 환경은 식물체의 생장, 개화시기, 동화산물 함량에 영향을 미치는 것으로 알려졌다. 본 연구는 고온 환경에서 감자의 생장과 대사산물 변화를 Soil-Plant-Atmosphere-Research (SPAR) 챔버와 온도구배하우스를 사용하여 2018년부터 2020년까지 조사하였다. 그 결과, 고온 조건의 SPAR 챔버 및 온도구배하우스에서 개화시기가 약 5∼9일 빨라지는 것을 확인하였다. 고온 조건은 감자 생장을 촉진하며 동화산물 함량에 영향을 줄 것으로 생각되어 개화 3일 전(Days Before Flowering 3, DBF 3)과 개화 21일 후(Days After Flowering 21, DAF 21)의 엽록소, 카로티노이드, 당 및 전분 함량을 분석하였다. 생육 초기인 DBF 3에 엽록소와 카로티노이드 함량은 감소하였고, 자당 및 전분 함량은 유의하게 증가하였다. 하지만 생육 후기인 DAF 21에는 자당 및 전분 함량이 감소한 것으로 나타났다. 또한 개화와 관련 있는 유전자 발현을 qRT-PCR로 분석한 결과, 고온 조건에서 SP6A, PhyB, SP5G, COL1, COL2 유전자 발현이 증가한 것을 확인하였다. 결과적으로, 고온 환경은 개화 전 감자 잎의 자당 및 전분 함량과 개화 관련 유전자의 발현에 영향을 미쳐 감자의 개화시기를 앞당기는 것으로 판단된다.

Outdoor storage fires have a significant impact on the surrounding environment, including adjacent storage facilities and buildings. Therefore, it is essential to review and manage the fire impact to minimize damage to human life and property on the outdoor storage fires. In this study, the heat release rate and radiant heat flux were simulated according to the fire time, wind veolcity, and presence or absence of water spray equipment in an outdoor storage facility fire, and the fire impact was analyzed. The outdoor storage was designed to simulate two scenarios on the outdoor storage fires containing gasoline, and FDS was used for fire simulation. As a results, when the water spray facility was not operating and the wind velocity was 5 m/s, the maximum radiant heat flux was 24.80 kW/m2, which exceeded the limit radiant heat flux of 20 kW/m2. When the water spray facility was operating and the wind veolcity was 10 m/s, the maximum radiant heat flux was 18.77 kW/m2, which did not exceed the limit radiant heat flux, indicating that the fire impact on adjacent storage facilities was relatively small.

고주파 흡수제(Accusorb MRI)를 이용한 자기공명영상 검사 중 화재가 발생했다. 다행히 환자는 화상을 입지는 않았 지만 스트랩과 4채널 코일의 케이블, Accusorb MRI가 녹아서 탄 흔적이 발견되었다. 자기공명영상 검사에 사용되 는 도구의 안전 지침을 준수해야 하지만 이를 지키지 않으면 화상, 열상, 장비 손상의 위험이 있다. 사고를 방지하기 위해서는 철저한 사전 점검을 해야 하고 모든 기기, 물품들이 자기공명영상 검사 시 사용 가능한 제품인지 확인하고 사용법을 정확하게 숙지하여야 한다.

This study aimed to determine the optimal planting density for sesame cultivation in a double cropping system after harvesting onion and garlic in the Muan region. It compared the growth, yield, disease susceptibility, and labor hours for two varieties of sesame (Landrace and Geonbaek sesame) at different planting densities. Plant height in the Landrace sesame showed no significant variation with different planting densities, whereas Geonbaek sesame increased in height with higher planting densities. Both cultivars increased the number of viable branches at lower densities. The stem diameter was thicker in Landrace sesame with decreasing planting density, while in the Geonbaek sesame, there was no significant difference in stem diameter regardless of planting density. The number of capsules per plant for the Landrace sesame increased with decreasing density, whereas Geonbaek sesame showed no significant difference. Yield for both cultivars was higher at greater densities. However, the late direct sowing time and shorter cultivation period significantly reduced the yield of sesame cultivated in a double cropping system compared to that of sesame grown in a single cropping system, due to the late direct sowing time and shorter cultivation period. There was no significant difference in disease occurrence based on planting density. Furthermore, although labor hours did not vary with planting density when comparing the labor hours required for cultivating the Landrace sesame and Geonbaek sesame, the latter needed less labor time. This study reveals the optimum planting distance and density for sesame cultivation as a double cropping after harvesting onion and garlic, providing invaluable data for establishing sesame double cropping cultivation techniques.

본 연구는 딸기의 삽목 육묘 시 묘소질을 향상시키고 품질을 증진시키기 위한 지베렐린 효과를 검증하기 위해 수행되었다. 품종은 ‘설향’을 대상으로 하였고, 삽목 시 삽수의 기부를 30, 60분간 GA3 50, 100, 150mg·L-1에 침지 처리하고 증류수 침 지를 대조구로 두었다. 정식 시 묘소질 조사 결과 엽수와 SPAD 값은 지베렐린 침지시간이 길수록 증가하였고, 초장, 엽병장, 엽장, 엽폭, 엽면적은 침지시간과 농도 간의 상호작용에 따라 유의하게 값이 변하였다. 관부직경은 같은 농도라 할지라도 침지시간에 따른 차이가 나타났으며 30분, 100mg·L-1 처리를 제외하고 모두 직경이 8.0mm 이상으로 우량한 묘가 생성되 었으며, T/R률은 30분, 50mg·L-1 처리에서 유의하게 작아 식 물체 활력이 가장 우수했다. 양자수율은 농도가 150mg·L-1일 때 저조하였고, 지베렐린 처리 농도가 높을수록 양자수율이 감소하는 경향을 나타냈다. NPQ는 대조구 대비 30분, 150mg·L-1 처리에서 유의하게 작아 묘 스트레스 감소에 효과 가 있었다. 항산화물 함량은 30분, 50mg·L-1 처리와 60분, 150mg·L-1 처리에서 높게 나타났고, 정식 후 생육조사 결과 화아분화 정도에 미치는 지베렐린의 부정적 영향은 없었다. 따라서, ‘설향’ 딸기의 삽목 육묘 시 지베렐린 처리는 정식묘 양성에 효과가 있는 것을 확인할 수 있었으며, 이때 대조구 대 비 생육 증진 및 품질 향상을 위해서는 삽수 기부를 50mg·L-1 의 지베렐린에 30분 침지처리하는 것이 보다 적정한 것으로 판단되었다.

This study was conducted to investigate the growth characteristics of domestic 'Sulhyang' strawberry (Fragaria × ananassa Duch.) seedlings and to analyze their relationships in order to develop a growth prediction model. Fresh weight, dry weight, and leaf area were measured to validate the newly developed growth model. The relative growth rate (RGR) of ‘Sulhyang’ seedlings’ dry weight was an average of 0.026 g·g-1·d-1, and it increased to 0.066 g·g-1·d-1 on the 49th day after transplanting (DAT). The relationship between DAT and RGR was represented as RGR (dry weight)(g·g- 1·d-1) = 0.0392/(1 + exp(–(DAT – 34.9940)/5.8662)). The crop growth rate (CGR) was an average of 0.060 g·m-2·d-1, and it increased to 0.211 g·m-2·d-1 on the 70th DAT. The relationship between DAT and CGR was calculated as CGR (dry weight)(g·m-2·d-1) = 0.1293/(1 + exp(–(DAT – 49.3917)/6.0928)). The relationship between shoot fresh weight (y) and shoot dry weight (x) per plant showed a linear relationship of y = 4.3189x + 0.7812 (R2 = 0.9976). Fresh weight, dry weight, and leaf area with respect to DAT and cumulative temperature increased exponentially, and sigmoid curve models were developed based on these data. The model with the highest coefficient of determination was found for the relationship between shoot dry weight (y) and cumulative temperature (x), represented as y = 14.2285/(1 + exp(–(x – 1590.1295)/377.8112)) (R2 = 0.9715). The results of this study can be utilized as valuable information for establishing a systematic management system for seedling production using strawberry cutting propagation methods. For the development of a more precise growth prediction model in the future, it is necessary to analyze and apply a wider range of growth indicators and meteorological factors related to strawberry seedlings.

This research was conducted to analyze the relationship between the induction time of runners and the growing degree days for domestic strawberry cultivars, 'Sulhyang' and 'Arihyang'. Runners were induced at 15-day intervals over five periods, starting from late April to early June, and the study compared the number of runner formations and temperature changes for each period while it also investigated the plantlet quality on the transplanting date. Based on back calculations from the transplanting date of September 18th, 'Sulhyang' required induction from late April to early May to achieve 70-day-old plantlets and from late April to mid-May for 60-day-old plantlets. On the other hand, 'Arihyang' needed induction from late April for 70-day-old plantlets and from late April to early May for 60-day-old plantlets. To secure about 20 daughter plants, the growing degree days needed to be around 1,000°C, and a delay in runner induction led to a delay in formation of daughter plants. The relationship between growing degree days and runner formation was expressed as the following quadratic equations: for 'Sulhyang,' y = 9E-06x2 + 0.0124x – 2.4806 (R2 = 0.9696), and for 'Arihyang,' y = 1E-06x2 + 0.0192x – 2.9274 (R2 = 0.9431), and these equations showed high correlations. The crown diameter on the transplanting date was thickest in mid-May for 'Sulhyang', and it tended to increase with delayed runner induction for 'Arihyang'. In conclusion, this study confirmed that the number of daughter plants and growth characteristics varied depending on strawberry cultivars and the timing of runner inducement time. The relationship equations derived through measurements of growing degree days can be used as the fundamental data for reasonable production of daughter plants in the future.

This study was conducted to identify the optimal root zone temperature for paprika cultivation, with an aim to increase the heating and cooling energy efficiency and prepare for extreme weather conditions. The greenhouse air temperature was maintained at 20oC and 25oC during the daytime (12 hours) and at 18oC during the nighttime (12 hours). The plant height did not show any significant differences between the treatment with air temperature and root zone temperature. The root length was highest under an air temperature of 25oC with root zone temperatures of 25oC and 30oC, and it was the lowest at 15oC. The leaf number was the highest when the root zone temperature was adjusted to 25oC and 30oC across all air temperatures. The leaf area increased with higher root zone temperatures, but considering the compactness of the seedlings, a root zone temperature of 25oC was found to be the most effective. The fresh and dry weight of the shoot increased with higher root zone temperatures at an air temperature of 25oC, while the fresh and dry weight of the roots tended to be higher when the root zone temperature was adjusted to 25oC and 30oC across all air temperatures. The compactness was most effective when the root zone temperature was adjusted to 20oC and 25oCC across all air temperatures. Based on the above results, adjusting the root zone temperature to 25oC at an air temperature of 25oC was found to be effective for the early growth of Paprika. The results of this study suggest that not only can growth be promoted through the regulation of root zone temperature, but it also contribute to the establishment of root zone temperature control technology, which can prevent an excessive drop and rise in the root zone temperature.

목적: 본 연구는 작업치료사가 시행한 시간 관련 중재의 효과와 임상 적용에 관하여 체계적으로 분석하였다. 연구방법: 2000년 1월부터 2022년 12월까지 학술연구정보서비스(Research Information Sharing Service), DBpia, PubMed, EMBASE, Web of Science 및 CINAHL을 통해서 ‘장애’, ‘시간관리’, ‘시간보조도구’, ‘중재’, ‘프로그램’, ‘작업치료’를 검색하였다. 해당 문헌들은 PRISMA-2020에 따라 체계적인 검토를 수행하였 고, PEDro를 통하여 연구의 질적 평가를 실시하였다. 최종 선정 기준에 부합한 연구는 총 9편이었다. 결과: 선정된 9편의 시간 관련 중재 중 시간기술훈련과 시간보조도구를 혼합하여 사용한 논문은 4편(44.5%), 시간보조도구는 3편(33.3%), 시간기술훈련은 2편(22.2%)이었다. 시간 관련 중재를 적용한 결과 시간기술에서 유의미한 효과를 보였다. 또한 인지 및 일상생활 기능, 작업 수행도 및 만족도, 심리 사회적 요인의 향상이 나타났다. 결론: 본 연구는 시간 관련 중재가 클라이언트의 시간 처리 능력 및 일상생활 수행 및 삶의 질 영역에 긍정적인 효과를 확인하였다. 추후 국내 작업치료 분야에서도 다양한 시간 관련 중재가 활용되기를 기대한다.

In this study, we developed Rapid Enrichment Broth for Vibrio parahaemolyticus (REB-V), a broth capable enriching V. parahaemolyticus from 100 CFU/mL to 106 CFU/mL within 6 hours, which greatly facilitates the rapid detection of V. parahaemolyticus. Using a modified Gompertz model and response surface methodology, we optimized supplement sources to rapidly enrich V. parahaemolyticus. The addition of 0.003 g/10 mL of D-(+)- mannose, 0.002 g/10 mL of L-valine, and 0.002 g/10 mL of magnesium sulfate to 2% (w/v) NaCl BPW was the most effective combination of V. parahaemolyticus enrichment. Optimal V. parahaemolyticus culture conditions using REB-V were at pH 7.84 and 37oC. To confirm REB-V culture efficiency compared to 2% (w/v) NaCl BPW, we assessed the amount of enrichment achieved in 7 hours in each medium and extracted DNA samples from each culture every hour. Real-time PCR was performed using the extracted DNA to verify the applicability of this REB-V culture method to molecular diagnosis. V. parahaemolyticus was enriched to 5.452±0.151 Log CFU/mL in 2% (w/v) NaCl BPW in 7 hours, while in REB-V, it reached 7.831±0.323 Log CFU/mL. This confirmed that REB-V enriched V. parahaemolyticus to more than 106 CFU/mL within 6 hours. The enrichment rate of REB-V was faster than that of 2% (w/v) NaCl BPW, and the amount of enrichment within the same time was greater than that of 2% (w/v) NaCl BPW, indicating that REB-V exhibits excellent enrichment efficiency.

This study compared the physicochemical properties of soybean curd residue and black soybean curd residue produced by hot air-drying and freeze-drying. Regardless of drying method, the crude protein, crude ash, crude fiber contents, pH, L, a, b color values and water soluble index were higher in soybean curd residue, whereas total polyphenol contents and antioxidant activity were higher in black soybean curd residue. Significant differences in water absorption index, oil absorption capacity and emulsion activity were observed between soybean curd residue and black soybean curd residue in freeze-drying. On the other hand, the emulsion stability was not significant difference in both hot-air drying and freezedrying. The crude protein and crude fiber contents of soybean curd residue were not significant difference between hot-air drying and freeze-drying. Freeze-drying resulted in higher crude ash contents, pH, water absorption index, water soluble index, oil absorption capacity, emulsion activity and emulsion stability than hot-air drying. Hot-air drying have caused significantly higher water contents, water activity, total polyphenol contents and antioxidant activity in soybean curd residue than freeze-drying. In conclusion, soybean type and drying methods affect the physicochemical and quality characteristics of soybean curd residue, which could be important factors in the manufacture of processed foods.

This study was conducted to determine the optimal dipping time and concentration of gibberellin for improving the growth and quality of domestic cultivar 'Seolhyang' strawberry when using runner plants. Strawberry runner plants were collected on November 10th and soaked in GA3 concentrations of 50, 100, and 150 mg·L-1 for 30 and 60 minutes, respectively. After 75 days of planting, the growth results showed that in the 30-minute, 50 mg·L-1 treatment, the crown diameter was thicker and the T/R ratio was lower, indicating better plant vitality. Runner length increased with lower gibberellin concentrations, particularly promoting vegetative growth. Photosynthetic efficiency was more influenced by gibberellin concentration than dipping time, and using concentrations above a certain threshold acted as a stress factor for runner plants, leading to decreased photosynthetic efficiency. For enhancing seedling growth, soaking with 50 mg·L-1 of gibberellin for 30 minutes was found to be optimal. This study verified the effects of gibberellin treatment on strawberry runner plants to improve plant growth and quality, providing useful basic data for using gibberellin.

본 연구의 목적은 지방도시재생 리빙랩을 위한 종합적이고 체계적인 평가지표를 마련하는 것이다. 특히 이 연구는 지방소멸의 전형적인 사례인 전주 물왕멀을 중심으로 이루어졌다. 전주의 도시재생 리빙랩인 물왕멀공동체의 이해관계자들을 대상으로 조사를 실시한 결과, 여러 평가 지표 중 특히 민주성에서도 주민의 참여비중과 주민 누구나 참여가능한지의 여부를 매우 중요하게 인식했다. 이는 향후 사업책임자의 선정 시 중요하게 평가되고 고려되어야 할 지표를 시사해 준다. 종합하면, 지방도시재생 리빙랩의 성패는 주요 이해관계자인 주민의 참여에 달려있다. 특히 노령인구의 비율이 높은 지방 축소도시의 특성상, 고령자의 눈높이에 적합한 사업을 선정하고 집행하는 한편 높은 전문성을 가진 사업책임자를 배치하여 진입장벽을 낮추는 데 세심하게 주의를 기울임으로써 주민의 참여욕구를 충족하는 것이 중요하다.

Objectives of this study were to propose an optimal fertilization method of Chlorella and to evaluate its effects on growth of green pepper. Chlorella fusca strain was propagated and used in the experiment. As for treatment, drenching method (DM), foliar fertilization (FF), drench and foliar fertilization combination (DM + FF), and untreated control (CO) were used. Plant height of green pepper was higher in the CO treatment than in chlorella fertilized treatments. Chlorella fertilization inhibited stem growth of green pepper. Chlorophyll contents of green pepper leaves were higher in the DM + FF treatment. Yields were the higher in DM and DM + FF treatments than in other treatments. Vitamin C content was the highest in the DM + FF treatment. These results showed that Chlorella fertilization could increase the growth, yield, and bioactive substance content of green pepper. The Chlorella fertilization method could be applied to other crops. By applying this technology to the farmer, it is possible to increase income and supply healthy and safe high-quality agricultural products to consumers.