This study was conducted to identify the optimal root zone temperature for paprika cultivation, with an aim to increase the heating and cooling energy efficiency and prepare for extreme weather conditions. The greenhouse air temperature was maintained at 20oC and 25oC during the daytime (12 hours) and at 18oC during the nighttime (12 hours). The plant height did not show any significant differences between the treatment with air temperature and root zone temperature. The root length was highest under an air temperature of 25oC with root zone temperatures of 25oC and 30oC, and it was the lowest at 15oC. The leaf number was the highest when the root zone temperature was adjusted to 25oC and 30oC across all air temperatures. The leaf area increased with higher root zone temperatures, but considering the compactness of the seedlings, a root zone temperature of 25oC was found to be the most effective. The fresh and dry weight of the shoot increased with higher root zone temperatures at an air temperature of 25oC, while the fresh and dry weight of the roots tended to be higher when the root zone temperature was adjusted to 25oC and 30oC across all air temperatures. The compactness was most effective when the root zone temperature was adjusted to 20oC and 25oCC across all air temperatures. Based on the above results, adjusting the root zone temperature to 25oC at an air temperature of 25oC was found to be effective for the early growth of Paprika. The results of this study suggest that not only can growth be promoted through the regulation of root zone temperature, but it also contribute to the establishment of root zone temperature control technology, which can prevent an excessive drop and rise in the root zone temperature.