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느타리버섯 품종 ‘흑타리’와 ‘미소’의 초위성체 특성구명 KCI 등재

Characterization of simple sequence repeats in the Pleurotus ostreatus cultivars, ‘Heuktari’ and ‘Miso’

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  • URLhttps://db.koreascholar.com/Article/Detail/320631
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한국버섯학회지 (Journal of Mushrooms (J. Mushrooms))
한국버섯학회 (The Korean Society of Mushroom Science)
초록

Simple sequence repeats (SSR), also referred to “microsatellites” consist of tandemly repeated short DNA sequence motifs and have been applied in various marker-based studies. SSRs were isolated and characterized from ‘Heuktari’ and ‘Miso’, which are major oyster mushroom cultivars in Korea, by genome sequencing and bioinformatic analysis. The genome sizes of ‘Heuktari’ and ‘Miso’ were estimated to be 40.8 and 40.3 Mb, respectively, which are larger than those of other P. ostreatus species (PC9 and PC10) and smaller than those of P. eryngii (KNR2312P5). In total, 949 and 968 SSRs were found in the ‘Heuktari’ and ‘Miso’ genomes, respectively. Comparative analysis of five mushrooms including P. ostreatus var. florida (PC9 and PC15) and P. eryngii revealed that the number of SSRs in ‘Heuktari’ and ‘Miso’ were the highest among them. All mushrooms studied showed similar SSR distribution patterns. Tri-, hexa-, and octanucleotide motifs accounted for the top three fractions of all SSRs.

저자
  • 박보경(경상남도농업기술원) | Bokyung Park
  • 하병석(경상남도농업기술원) | Byeong Seok Ha
  • 김민근(경상남도농업기술원) | Min Keun Kim
  • 이병주(충북농업기술원) | Byungjoo Lee
  • 최종인(경기도농업기술원) | Jong In Choi
  • 류재산(경상남도농업기술원) | Jae-San Ryu