This study was accomplished to investigate the effects of the night temperatures on the dark respiration and fruit quality of ‘Heukgoosul’ grape at ripening time. The weight of cluster and berry increased significantly at 20oC than 30oC in night temperature. The length and diameter of berry harvested from the 20oC and 25°C treatments also increased significantly. But there were no differences in the cluster length and diameter as well as the number of berries per cluster. When the amount of CO2 uptake was examined, the dark respiration decreased at 20°C in comparison with 30°C. On the other hand, the anthocyanin content of fruits harvested from the 20°C treatment was higher 38.8 μg·cm-2 than that of 30°C 27.7 μg·cm-2. Hunter’s value such as L(brightness), a(red chromaticity) and b(yellow chromaticity) showed the significance between 20°C and 30°C treatments. Soluble solid content and acidity of berry fruits harvested from the 20°C treatment increased respectively by 3.4 °Brix and 1.6 times. With consideration of the main characteristics of fruit harvested at 30°C, the sugar-acid ratio 72.6% was lowered and the color chart showed the low value 7.1, therefore, the overall fruit quality was deteriorated. When the production yield rates of first harvest 23.6% and second harvest 44.7% were compared, the 20°C treatment could move up the harvest time earlier.