Alcoholic steatosis is a fundamental metabolic disorder and may precede the onset of more severe forms of alcoholic liver disease. In this study, we isolated enzymatichydrolysate from Semisulcospira libertine by alcalase hydrolysis and investigated the protective effect of Semisulcospira libertine hydrolysate on liver injury induced by alcohol in the mouse model of chronic and binge ethanol feeding (NIAAA). In an in vitro study, the hydrolysate protects HepG2 cells from ethanol toxicity. Liver damage was assessed by histopathological examination, as well as by quantitating activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). After the administration of S. libertina hydrolysate, fat accumulation and infiltration of inflammatory cells in liver tissues were significantly decreased in the NIAAA mouse model. The elevated levels of serum AST, ALT, and ALP activities, along with the lipid contents of a damaged liver, were recovered in experimental mice administrated with S. libertina hydrolysate, suggesting its role in blood enzyme activation and lipid content restoration within damaged liver tissues. Moreover, treatment with S. libertine hydrolysate reduced the expression rate of cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-6, which accelerate inflammation and induces tissue damage. All data showed that S. libertine hydrolysate has a preventive role against alcohol-induced liver damages by improving the activities of blood enzymes and modulating the expression of inflammation factor, suggesting S. libertine hydrolysate could be a commercially potential material for the restoration of hepatotoxicity.

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 References

• 송은진(경상대학교 의과대학 외과학교실 건강과학연구원, 경상대학교병원 의생명연구원, Department of Surgery, Gyeongsang National University School of Medicine, Institute of Health Sciences, Biomedical Research Institute, Gyeongsang National University Hospital) | Eun Jin Song
• 조경환((재)하농녹차연구소, Institute of Hadong Green Tea) | Kyoung Hwan Cho
• 추호진((주)정옥, Jeongok Co. Ltd.) | Ho Jin Choo
• 양은영((주)정옥, Jeongok Co. Ltd.) | Eun Young Yang
• 정윤경((주)정옥, Jeongok Co. Ltd.) | Yoon Kyoung Jung
• 서민균((주)정옥, Jeongok Co. Ltd.) | Min Gyun Seo
• 김종철((재)하농녹차연구소, Institute of Hadong Green Tea) | Jong Cheol Kim
• 강은주((재)하농녹차연구소, Institute of Hadong Green Tea) | Eun Ju Kang
• 류기형(공주대학교 식품공학과, Department of Food Science and Technology, Kongju National University) | Gi Hyung Ryu
• 박범용(경상대학교 의과대학 외과학교실 건강과학연구원, 경상대학교병원 의생명연구원, Department of Surgery, Gyeongsang National University School of Medicine, Institute of Health Sciences, Biomedical Research Institute, Gyeongsang National University Hospital) | Beom Yong Park
• 하영술(경상대학교 의과대학 외과학교실 건강과학연구원, 경상대학교병원 의생명연구원, Department of Surgery, Gyeongsang National University School of Medicine, Institute of Health Sciences, Biomedical Research Institute, Gyeongsang National University Hospital) | Young-Sool Hah Corresponding author