Background: Adenophora triphylla var. japonica (Regel) H. Hara shows vegetative growth with radical leaves during the first year and shows reproductive growth with cauline leaves and bolting during the second year. In addition, the shape of the plant varies within the same species. For this reason, there are limitations to classifying the species by visual examination. However, there is not sufficient genetic information or molecular tools to analyze the genetic diversity of the plant. Methods and Results: Approximately 34.59 Gbp of raw data containing 342,487,502 reads was obtained from next generation sequencing (NGS) and these reads were assembled into 357,211 scaffolds. A total of 84,106 simple sequence repeat (SSR) regions were identified and 14,133 primer sets were designed. From the designed primer sets, 95 were randomly selected and were applied to the genomic DNA which was extracted from five plants and pooled. Thirty-nine primer sets showing more than two bands were finally selected as SSR markers, and were used for the genetic relationship analysis. Conclusions: The 39 novel SSR markers developed in this study could be used for the genetic diversity analysis, variety identification, new variety development and molecular breeding of A. triphylla.

ABSTRACT
 서 언
 재료 및 방법
  1. SSR 구간 탐색 및 프라이머 제작
  2. 다형성 SSR 프라이머 선발
  3. SSR 마커의 효율성 검증
 결과 및 고찰
  1. 염기서열 분석 및 SSR 구간 탐색과 프라이머 제작
  2. 다형성을 보이는 SSR 프라이머 선발
  3. SSR 마커의 효율성 검증
 감사의 글
 REFERENCE

• 박기찬(농촌진흥청 국립원예특작과학원 인삼특작부, Department of Herbal Crop Research, NIHHS, RDA) | Ki Chan Park
• 김영국(농촌진흥청 국립원예특작과학원 인삼특작부, Department of Herbal Crop Research, NIHHS, RDA) | Young Guk Kim
• 황보경(충북대학교 특용식물학과, Department of Industrial Plant Science and Technology, Chungbuk National University) | Kyeong Hwangbo
• 길진수(충북대학교 특용식물학과, Department of Industrial Plant Science and Technology, Chungbuk National University) | Jinsu Gil
• 정희(충북대학교 특용식물학과, Department of Industrial Plant Science and Technology, Chungbuk National University) | Hee Chung
• 박신기(㈜테라젠이텍스 바이오연구소, Theragen Etex Bio Institute) | Sin Gi Park
• 홍창표(㈜테라젠이텍스 바이오연구소, Theragen Etex Bio Institute) | Chang Pyo Hong
• 이이(충북대학교 특용식물학과, Department of Industrial Plant Science and Technology, Chungbuk National University) | Yi Lee Corresponding author