Background: In the herbal medicinal industry, Angelica gigas Nakai, Angelica sinensis (Oliv.) Diels. and Angelica acutiloba (Siebold & Zucc.) Kitag. are often confused, because the roots of the three species can not be distinguished by their appearance. This confusion can cause serious side effects. In this study, we determined the origins of Angelica roots distributed in the Korean market using the simple sequence repeat (SSR) markers developed based on the A. gigas chloroplast DNA sequence. Methods and Results: We collected twenty seven A. gigas and three A. acutiloba samples from the Seoul, Daegu, and Cheongju herbal medicinal markets. Fifty sections of one collection were mixed and ground to make a powder, which was used for DNA extraction using the cetyl trimethylammonium bromide (CTAB) method. Chloroplast based SSR markers were applied to the DNA for the determination of the species. In addition, polymorphism was found in eight samples. The phylogenetic analysis showed that the A. gigas roots collected from herbal medicinal markets were clearly discriminated from A. sinensis and A. acutiloba even though they were grouped into four clusters. Conclusions: This study showed that chloroplast based SSR markers would help the discrimination of Angelica roots in the Korean herbal medicinal industry and the markers are useful to prevent confusion between Angelica roots.

ABSTRACT
 서 언
 재료 및 방법
  1. 당귀 종판별 마커
  2. 시료 수집 및 마커 적용
  3. 유전적 다양성 및 유연관계 분석
 결과 및 고찰
  1. 수집된 당귀의 기원 판별
  2. 다형성을 보이는 참당귀 시료의 분석
  3. 분자마커를 이용한 기원판별
 감사의 글
 REFERENCES

• 박상익(충북대학교 특용식물학과, Department of Industrial Plant Science and Technology, Chungbuk National University) | Sang Ik Park
• 황보경(충북대학교 특용식물학과, Department of Industrial Plant Science and Technology, Chungbuk National University) | Kyeong Hwangbo
• 길진수(충북대학교 특용식물학과, Department of Industrial Plant Science and Technology, Chungbuk National University) | Jinsu Gil
• 정희(충북대학교 특용식물학과, Department of Industrial Plant Science and Technology, Chungbuk National University) | Hee Chung
• 김호방((주)바이오메딕 생명과학연구소, Life Sciences Research Institute, Biomedic Co., Ltd) | Ho Bang Kim
• 김옥태(농촌진흥청 국립원예특작과학원 인삼특작부, Department of Herbal Crop Research, NIHHS, RDA) | Ok Tae Kim
• 김성철(농촌진흥청 국립원예특작과학원 인삼특작부, Department of Herbal Crop Research, NIHHS, RDA) | Seong Cheol Kim
• 구성철(농촌진흥청 국립원예특작과학원 인삼특작부, Department of Herbal Crop Research, NIHHS, RDA) | Sung Cheol Koo
• 엄유리(국립산림과학원 산림약용자원연구소, Forest Medicinal Resources Research Center, National Institute of Forest Science) | Yurry Um
• 이이(충북대학교 특용식물학과, Department of Industrial Plant Science and Technology, Chungbuk National University) | Yi Lee Corresponding author