This study was performed to investigate the understanding and recognition status on separation anxiety in dogs. Most breeders(95% of the survey respondents) knew about separation anxiety in dogs, but they often neglected their dogs with no treatment and no taking care of them. Symptoms of separation anxiety are mental anxiety conditions, such as severely barking, disturbing house littered, defecation mistake and self-injury. Breeders may suffer the hardships with their dogs having separation anxiety. So the efforts are needed to reduce the occurrence of separation anxiety, and it is desirable for patients to seek animal behavior counseling, behavioral correction training, and medication therapy.