This study was designed to evaluate the antioxidant activities and protective effects on PC12 cells of the extract of Epimedium koreanum and its main constituents icariin and icariside I. After screening the seven identified flavonoid glycosides from E. koreanum through DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay, E. koreanum, Icariin and Icariside I exhibited significant effect on radical scavenging activity. E. koreanum, icariin and icariside I were examined using DPPH, ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) and FRAP (ferric reducing ability power) assay. In all antioxidant assays, E. koreanum, icariin and icariside I showed high radical scavenging activities in a dose-dependent manner. Protective effects against H2O2-induced PC12 cells were assessed with MTT assay. The results indicated that cell viability and protection on PC12 cells of icariside I and icariin increased dose dependently. These study results suggest that E. koreanum, icariin and icariside showed high antioxidant capacities and cell protective effects. Icariside I, one of the metabolites of icariin, may be a new and effective flavonoid compound as a functional component.

I. 서 론
 II. 연구 내용 및 방법
  1. 실험 재료
  2. 실험 농도
  3. DPPH 라디칼 소거 활성
  4. ABTS 라디칼 소거 활성
  5. Ferric reducing antioxidant power (FRAP)
  6. 세포 배양
  7. 세포 내 산화적 스트레스 억제 효과
 III. 결과 및 고찰
  1. DPPH 라디칼 소거 활성
  2. ABTS 라디칼 소거 활성
  3. FRAP
  4. 세포 독성 확인 및 산화적 스트레스에 대한 세포 보호 효과
 IV. 결론 및 요약
 References

• 유소현(덕성여자대학교 식물자원연구소, Plant Resources Research Institute, Duksung Women’s University, Seoul) | SoHyeon You
• 김건희(덕성여자대학교 식물자원연구소, Plant Resources Research Institute, Duksung Women’s University, Seoul) | Gun-Hee Kim Corresponding author