HPLC-size-exclusion chromatography에 의해 가공식품에서 알긴산프로필렌글리콜의 함량을 분석하는 방법이 개발 되었다. 알긴산프로필렌글리콜을 분석하기 위해 GF-7M HQ column과 LT-ELSD detector가 선정되었다. 알긴산프 로필렌글리콜 분석을 위한 전처리 조건으로는 20oC에서 150 rpm으로 3시간 동안 추출하는 방법이 선정되었다. 알긴산프로필렌글리콜을 5 농도(300, 500, 700, 1,000, and 1,500 mg/kg) 범위에서 검량선을 작성한 결과 직선성(R2) 은 0.9873으로 측정되었다. HPLC system에 의한 알긴산 프로필렌글리콜 분석시 검출한계(LOD) 및 정량한계(LOQ) 는 각각 171.43 mg/kg 및 519.50 mg/kg이었다. Size-exclusion chromatography에 의해 얻은 회수율 및 변동 계수(coefficient of variation)는 각각 86.1~110.4% 및 4.1~13.5% 이었다. 본 연구에서 개발된 HPLC-size-exclusion chromatography system을 적용하여 134 품목의 가공식품에서 알긴 산프로필렌글리콜 함량을 분석하였다. 이 결과들은 이 방법이 가공식품에서 알긴산프로필렌글리콜 함량을 분석하는데 적용할 수 있는 방법이라는 것을 나타낸다.

An analytical method for determination of propylene glycol alginate (PGA) in food products was developed by HPLC-size-exclusion chromatography. The GF-7M HQ column and LT-ELSD detector were determined by considering the instrumental analysis conditions for PGA analysis. The pretreatment method for the analysis of PGA was suitable for 3 hr extraction at 20oC and 150 rpm according to the extraction temperature. Linearity (R2) for the analysis of PGA was 0.9873 at calibration curve range of 300, 500, 700, 1,000, and 1,500 mg/kg (5 points). The limit of detection and limit of quantification of PGA on HPLC system was 171.43 and 519.50 mg/kg, respectively. The accuracy and coefficient of variation obtained by size-exclusion chromatography were 86.1~110.4% and 4.1~13.5%, respectively. By applying the HPLC-size-exclusion chromatography system, it was possible to analyze the contents of PGA in 134 different types of food products.

Materials and Methods
  시약 및 장비
  Column 및 분석법 확립
  시료 전처리법 확립
  분석법 검증
  표준용액
  시료 전처리
  통계처리
 Results and Discussion
  표준품 용해 용매선정
  HPLC를 이용한 알긴산프로필렌글리콜 분석조건 확립
  시료 전처리 방법
  분석법 검증
  식품 중 알긴산프로필렌글리콜 함량 분석
 Acknowledgement
 국문요약
 References

• 정은정(전북대학교 식품공학과, Department of Food Science & Technology, Chonbuk National University, Jeonju, Korea) | Eun-Jeong Jeong
• 최유정(전북대학교 식품공학과, Department of Food Science & Technology, Chonbuk National University, Jeonju, Korea) | Yoo-Jeong Choi
• 이근영(식품의약품안전평가원 첨가물포장과, Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation, Osong, Korea) | Gunyoung Lee
• 윤상순(식품의약품안전평가원 첨가물포장과, Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation, Osong, Korea) | Sang Soon Yun
• 임호수(식품의약품안전평가원 첨가물포장과, Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation, Osong, Korea) | Ho Soo Lim
• 김용석(전북대학교 식품공학과, Department of Food Science & Technology, Chonbuk National University, Jeonju, Korea) | Yong-Suk Kim Correspondence to